Journal of Diabetes Research / 2019 / Article / Fig 3

Research Article

Visfatin Induces Inflammation and Insulin Resistance via the NF-κB and STAT3 Signaling Pathways in Hepatocytes

Figure 3

Visfatin activated NF-κB and STAT3 signaling in HepG2 cells. (a) HepG2 cells were incubated with 200 ng/mL visfatin for the indicated times. Inhibitory insulin signaling was analyzed by immunoblotting for phospho-IRS-1 (p-IRS-1, Ser 307) and total IRS-1. compared to the untreated control. (b) JAK/STAT3 signaling was analyzed using anti-phospho-JAK2, anti-phospho-STAT3, and anti-actin antibodies. and compared to the untreated control. (c) IKK/NF-κB signaling was analyzed using anti-phospho-IKKα/β, anti-phospho-NF-κB, IκBα, and anti-actin antibodies. , , and compared to the untreated control. (d) MAP kinase signaling was analyzed using anti-phospho-JNK, anti-phospho-ERK, anti-phospho-p38, and anti-actin antibodies. The maximum control phosphoprotein intensities were set to 100%, and relative test intensities were then calculated. The data are of those of three independent experiments.
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