Pioglitazone Ameliorates Atorvastatin-Induced Islet Cell Dysfunction through Activation of FFA1 in INS-1 Cells

Figure 2

Effect of atorvastatin on the expression of FFA1, PDX-1, and NeuroD in INS-1 cells. (a) qRT-PCR assay analysis of FFA1 expression in INS-1 cells treated with different concentrations of atorvastatin for 24 h. (b and c) Western blot assay analysis of FFA1 expression in INS-1 cells treated with different concentrations of atorvastatin for 24 h. (d) qRT-PCR assay analysis of PDX-1 expression in INS-1 cells treated with different concentrations of atorvastatin for 24 h. (e) qRT-PCR assay analysis of BETA2/NeuroD expression in INS-1 cells treated with different concentrations of atorvastatin for 24 h. (f, g, and h) Western blot assay analysis of PDX-1 and BETA2/NeuroD expression in INS-1 cells treated with different concentrations of atorvastatin for 24 h. β-Actin was detected as control. Each experiment was repeated at least three times. and compared to 0 μM atorvastatin treatment.

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