HNF1α Controls Liver Lipid Metabolism and Insulin Resistance via Negatively Regulating the SOCS-3-STAT3 Signaling Pathway

Figure 6

STAT3 inhibitor NSC74859 can reverse the effect of the downregulation of HNF1α expression on hepatic glycolipid metabolism. (a) Oil Red O staining test showed that NSC74859 reduced the number of red granular lipid droplets in LO2 cells induced by FFA. Downregulation of HNF1α expression followed by addition of NSC74859 reduced FAR-induced red granule lipid droplets in LO2 cells. (b) Biochemical indicators showed that NSC74859 reduced TC, TG, and NEFA contents. After downregulating the HNF1α expression and adding NSC74859, the contents of TC, TG, and NEFA decreased significantly. (c, d) Western blot analysis showed that NSC74859 promoted IRS-1 and AKT phosphorylation. Downregulation of HNF1α expression followed by addition of NSC74859 abolished the inhibition of IRS-1 and AKT phosphorylation by downregulating the HNF1α expression. (e) Western blot analysis showed that NSC74859 inhibited the expression of SREBP-1c and promoted the expression of PPARα. Downregulation of HNF1α expression followed by NSC74859 reversed the effects of downregulation of HNF1α expression on SREBP-1c and PPARα expression. Reported values are the of the three independent tests, with , , and .

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