Advanced Glycation End-Products and Hyperglycemia Increase Angiopoietin-2 Production by Impairing Angiopoietin-1-Tie-2 System

Figure 2

AGEs and HG did not alter the expression and phosphorylation of Tie-2. (a) Western immunoblotting of Tie-2 in HMEC-1 cells cultured for 5 days in CTR, AGEs, HG, and HG+AGEs. (b) After the 5-day treatment described above, HMEC-1 cells were incubated for 2 hours in serum-free medium and, then, exposed for 30 min to PBS (control) or Ang-1 (200 ng/ml). Then, Tie-2 and phosphoTie-2 were analyzed. Representative western blot analysis and quantification of densitometries of western blot band. Data were expressed as of fold induction relative to β-actin (a) or to relative expression of Tie-2 and phTie-2 (b) (). and vs. same culture condition in the absence of Ang-1.

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