Research Article

Advanced Glycation End-Products and Hyperglycemia Increase Angiopoietin-2 Production by Impairing Angiopoietin-1-Tie-2 System

Figure 3

AGEs and HG reduced phosphorylation of AKT and FoxO1 induced by Ang-1. After the 5-day treatment described above, HMEC-1 cells were incubated for 2 hours in serum-free medium and, then exposed for 30 min to PBS (control) or Ang-1 (200 ng/ml). Then, phAKT and AKT (a) and phFoxO1 and FoxO1 (b) were analyzed. Representative western blot analysis and quantification of densitometries of western blot band. Data were expressed as of fold induction relative to unphosphorylated protein (). vs. the same culture condition in the absence of Ang-1; $ and $$$ vs. CTR+Ang-1; and +++ HG+AGEs+Ang-1 vs. HG+Ang-1.
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