Inhibition of Aberrant IGF-I Signaling in Diabetic Male Rat Retina Prevents and Reverses Changes of Diabetic Retinopathy

Figure 8

Disruption of IAP/SHPS-1 association via intraocular injection of an anti-IAP antibody attenuated IGF-I-stimulated AKT activation and VEGF expression in the retinas from diabetic rats. Animals ( for nondiabetic rats, for diabetic rats with control antibody, and for diabetic rats with IAP antibody) were treated, and retinal extracts were prepared following the protocol described in Materials and Methods. The retinal extracts were immunoblotted with an anti-pAKT (a) or VEGF (b) antibody. The blots were reprobed with anti-β-actin antibody as a loading control. The bar graphs show the ratio of scan values of pAKT band or VEGF band divided by β-actin band. indicates the significant difference between two treatments. , NS indicates no significant difference. (c) The retinal extracts were immunoblotted with an anti-VWF antibody. The blots were reprobed with anti-β-actin antibody as a loading control.

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