Karyopherin Alpha 2-Expressing Pancreatic Duct Glands and Intra-Islet Ducts in Aged Diabetic C414A-Mutant-CRY1 Transgenic Mice

Figure 4

Coimmunostaining of islets for insulin and KPNA2. Pancreas sections from WTs (upper panels) and TGs (lower panels) of late middle age were costained with antibodies to insulin (green) and KPNA2 (red) and counterstained with 4,6-diamidino-2-phenylindole (DAPI; blue) for nuclear staining. Bar, 20 μm. KPNA2 was located abundantly in the nuclei of β-cells in WTs. In TGs, insulin-positive cells were far fewer than in WTs. Even in the remaining β-cells of TGs, KPNA2 expression was observed (a typical cell is denoted with a yellow triangle).