Melatonin Affects Mitochondrial Fission/Fusion Dynamics in the Diabetic Retina

Figure 2

Melatonin treatments alleviate HG-induced decreases in mitochondrial Ca²⁺ buffering. Calcium-imaging of cultured 661W cells after 24 h of treatments with H₂O (CON), HG, or HG+melatonin (HG+M). Cells were loaded with Fluo-4 and Rhod-2 for cytosolic and mitochondrial Ca²⁺ imaging, respectively. Scale  μm in (a) and 10 μm in (b). The Rhod-2 (c) and Fluo-4 (d) fluorescent intensities indicating mitochondrial (c) and cytosolic (d) Ca²⁺ were quantified. The experiments were repeated at least three times. .

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