FXR Mediates Adenylyl Cyclase 8 Expression in Pancreatic β-Cells

Figure 2

Expression of FXR in islets from Wistar and GK rats. (a, b) Cytosol and nuclear proteins extracted from Wistar and GK rat pancreases were subjected to western blot analysis. Histone3 and β-actin were used as nuclear and cytosol internal control, respectively. Data are . ; . (c) Representative immunostaining images for FXR (green), insulin (red), DAPI (blue), and merge of the three in single β-cells isolated from Wistar and GK rats; . Scale bars represent 5 μm. (d) Statistical analysis of FXR expression in β-cells isolated from Wistar and GK rats. Data are . ; . (e) FXR protein expression in Wistar and GK rat islets. Data represent . ; . (f) Insulin secretion in islets isolated from Wistar and GK rats. The islets were treated with 16.8 mM glucose in the presence of DMSO or 5 μM GW4064 for 30 min. Data are . ; .

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