Research Article

FXR Mediates Adenylyl Cyclase 8 Expression in Pancreatic β-Cells

Figure 4

FXR directly binds to the Adcy8 promoter. (a) A putative FXR binding site in the Adcy8 promoter of different species. (b) 293T cells were transfected with a luciferase reporter driven by the Adcy8 promoter, followed by treatment with 5 μM GW4064 for 24 h prior to luciferase activity analysis for the reporter expression. Data are . ; . ChIP analysis for FXR occupancy in the promoter of Adcy8 in INS-1 832/13 cells treated with 5 μM GW4064 for 24 h (c) or in FXR knockdown INS-1 832/13 cells (d). Data are of three independent experiments. .
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