Journal of Diabetes Research / 2020 / Article / Fig 2

Research Article

3β-Hydroxysteroid-Δ24 Reductase (DHCR24) Protects Pancreatic β Cells from Endoplasmic Reticulum Stress-Induced Apoptosis by Scavenging Excessive Intracellular Reactive Oxygen Species

Figure 2

Apoptosis of MIN6 cells induced by TM is inhibited by overexpression of DHCR24. (a) A representative fluorescent image of cleaved caspase-3 (green) was obtained by IC analysis 24 h after TM exposure. Scale bar, 50 μm. (b) The relative fluorescence intensity of cleaved caspase-3 was analyzed with ImageJ software. (); vs. Ad-LacZ. (c) The apoptosis of MIN6 cells was analyzed by TUNEL 24 h after TM treatment. The green fluorescent signal represents TUNEL-positive cells. Scale bar, 100 μm. (d) The TUNEL-positive cell number was calculated as percentage of TUNEL-positive cell numbers to adherent cells in the same field. (); vs. Ad-LacZ.

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