3<i>β</i>-Hydroxysteroid-<i>Δ</i>24 Reductase (DHCR24) Protects Pancreatic <i>β</i> Cells from Endoplasmic Reticulum Stress-Induced Apoptosis by Scavenging Excessive Intracellular Reactive Oxygen Species

<div>Apoptosis of MIN6 cells induced by TM is inhibited by overexpression of DHCR24. (a) A representative fluorescent image of cleaved caspase-3 (green) was obtained by IC analysis 24 h after TM exposure. Scale bar, 50 <i>μ</i>m. (b) The relative fluorescence intensity of cleaved caspase-3 was analyzed with ImageJ software. <svg height="9.10327pt" id="M18" style="vertical-align:-0.3499298pt" version="1.1" viewbox="-0.0498162 -8.75334 64.9562 9.10327" width="64.9562pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M861 0V28C774 35 771 41 768 147L759 509C756 612 762 614 851 622V650H681L449 149L221 650H57V622C148 613 153 609 144 479L130 271C123 166 117 123 111 88C104 46 85 34 26 28V0H259V28C192 35 169 42 167 90C166 130 166 173 170 256L185 541H187L411 7H431L675 555H679L683 147C683 41 680 35 598 28V0H861Z"></path></g><g transform="matrix(.013,0,0,-0.013,11.244,0)"><path d="M380 106C343 72 306 56 265 56C195 56 116 112 115 248C235 252 361 262 377 265C396 269 400 277 400 297C400 374 333 449 250 449H249C198 449 144 421 103 376S37 269 37 201C37 88 109 -12 232 -12C263 -12 332 6 395 84L380 106ZM225 412C281 412 315 364 314 312C314 297 308 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(c) The apoptosis of MIN6 cells was analyzed by TUNEL 24 h after TM treatment. The green fluorescent signal represents TUNEL-positive cells. Scale bar, 100 <i>μ</i>m. 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Journal of Diabetes Research

fig2

Figure 2

Figure 2: 3<i>β</i>-Hydroxysteroid-<i>Δ</i>24 Reductase (DHCR24) Protects Pancreatic <i>β</i> Cells from Endoplasmic Reticulum Stress-Induced Apoptosis by Scavenging Excessive Intracellular Reactive Oxygen Species 

Figure 2 | 3β-Hydroxysteroid-Δ24 Reductase (DHCR24) Protects Pancreatic β Cells from Endoplasmic Reticulum Stress-Induced Apoptosis by Scavenging Excessive Intracellular Reactive Oxygen Species 