Protective Role of Tangshen Formula on the Progression of Renal Damage in db/db Mice by TRPC6/Talin1 Pathway in Podocytes

Figure 3

TSF protects podocyte-associated protein talin1 in db/db mice, which may involve increased expression of TRPC6 in podocytes. (a) Immunoblotting and quantitative analysis of talin1 ( experiments). (b) Immunofluorescence costaining (scale bars, 25 μm) of nephrin (red) and talin1 (green) showed that talin1 had a specific deletion in podocytes. (c) Immunofluorescence costaining (scale bars, 25 μm) of nephrin (red; red arrow) and TRPC6 (green) showed that increased expression of podocyte TRPC6 in db/db mice (yellow arrow) compared to db/m mice and db/db + TSF mice (). Fluorescent images were collected and assessed using a high-content screening system. The data were expressed as the . ^#, ^## vs. db/m group; , vs. db/db group. Statistically analyzed via a one-way ANOVA with Dunnett’s correction.

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