Geranylgeranyl Transferase-I Knockout Inhibits Oxidative Injury of Vascular Smooth Muscle Cells and Attenuates Diabetes-Accelerated Atherosclerosis
Effects of GGTase-I knockout on high glucose (22.2 mM) induced proliferation and ROS generation of VSMCs. VSMCs were isolated from wild-type and Pggt1bΔ/Δ mice and treated with normal glucose (5.6 mM) or high glucose (22.2 mM) for 72 h. VSMCs from wild-type mice were also cultured with a selective GGTase-I inhibitor (GGTI-286, 10 μM) or Rac1 inhibitor (100 μM) for 24 h. Cell proliferation was assessed by MTT incorporation assay (a). ROS production in VSMCs was measured by Amplex Red (b) and carboxy-H2DCFDA (c) chemiluminescence, respectively. Data are expressed as , for each group. versus the strain-matched normal glucose group. # and ## versus the wild-type high-glucose group.
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