Research Article

Sirt1 Mediates Vitamin D Deficiency-Driven Gluconeogenesis in the Liver via mTorc2/Akt Signaling

Figure 4

1,25(OH)2D3 deficiency inhibits the expression of Sirt1, a target gene of VDR/RXR, which then has an inhibitory effect on the Rictor expression. (a) Bioinformatics prediction of binding sites between VDR and Sirt1 (the first and second binding regions in Sirt1 promoter are VDR potential target binding sites and the third binding region in Sirt1 promoter is RXR potential target binding sites). (b, c) mRNA (b) and protein (c) expression levels of Sirt1 in the liver samples collected from wild-type and 1α(OH)ase-/- mice with rescued diet. . (d) IHC was employed to detect the Sirt1 expression in the livers of wild-type and 1α(OH)ase-/- mice with rescued diet. . (e, f) mRNA (e) and protein (f) expression levels of Sirt1 after transfection of si-NC or si-VDR in HepG2 cells. ; blue bar: si-NC group; red bar: si-VDR group. (g, h) mRNA (g) and protein (h) expression levels of Rictor in the livers from wild-type and 1α(OH)ase-/- mice with rescued diet. , RDWT vs. RDKO. (i, j) mRNA (i) and protein (j) expression levels of Rictor after transfection of si-NC or si-VDR in HepG2 cells. , si-NC vs. si-VDR; blue bar: si-NC group; red bar: si-VDR group.
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