Research Article

Partial Purification and Characterization of the Mode of Action of Enterocin S37: A Bacteriocin Produced by Enterococcus faecalis S37 Isolated from Poultry Feces

Figure 1

Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (Tricine-SDS-PAGE). (a) Silver-stained gel. Lane 1: molecular weights markers; lane 2: culture supernatant of E. faecalis S37; lane 3: heat and filtered supernatant culture of E. faecalis S37; lane 4: active fraction collected after ammonium sulfate precipitation; lane 5: active peak obtained after first RP-HPLC; lane 6: active peak obtained after second RP-HPLC. (b) The gel was overlaid with L. monocytogenes EGDe to determine the antimicrobial activity of the purified enterocin S37; lane 7: active peak obtained after first RP-HPLC; lane 8: active peak obtained after second RP-HPLC. (c) Agar diffusion test. Well 1: negative control (phosphate buffer); well 2: heated and filtered supernatant culture of E. faecalis S37; well 3: active fraction collected after ammonium sulfate precipitation; well 4: active peak obtained after first RP-HPLC; well 5: active peak obtained after second RP-HPLC.
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