Research Article

Protease from Aspergillus oryzae: Biochemical Characterization and Application as a Potential Biocatalyst for Production of Protein Hydrolysates with Antioxidant Activities

Table 3

Kinetic parameters for the protease from A. oryzae LBA 01 produced under solid state fermentation of wheat bran.

Kinetic activation parameters
Temperature range (°C) (kJ moL−1)

30–5537.980.991.64–1.53
55–80−94.870.95

Michaelis-Menten constant () and maximum rate for the enzymatic reaction ()
Substrate (mg·mL−1) (U·g−1)

Casein4.95446.3
Azocasein2.55139.3
Hemoglobin0.72764.5

Kinetic parameters for irreversible thermal denaturation
Temperature (°C) (min−1) (min) (min)

57.20.007197.63324.310.92