Table of Contents
Journal of Food Processing
Volume 2015 (2015), Article ID 609703, 8 pages
Research Article

Optimizing the Extraction of Dietary Fibers from Sorghum Bran Using Response Surface Methodology

1Department of Biochemistry, Faculty of Science, University of Yaounde I, P.O. Box 812, Yaounde, Cameroon
2Department of Life and Earth Sciences, Higher Teachers’ Training College, University of Maroua, P.O. Box 55, Maroua, Cameroon
3Department of Chemistry, Faculty of Science, University of Maroua, P.O. Box 814, Maroua, Cameroon

Received 5 August 2015; Revised 12 September 2015; Accepted 17 September 2015

Academic Editor: Xuetong Fan

Copyright © 2015 Ange-Patrice Takoudjou Miafo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Response surface methodology was used to optimize the processing parameters of the fiber extraction from sorghum bran. The studied independent factors were ethanol/bran ratio, time, temperature, and number of treatment cycles. A three-level four-variable Box-Behnken design (BBD) was used to establish the optimum conditions of extraction. The results showed that the experimental data could be fitted to a second-order polynomial equation using multiple regression analysis and the model was highly significant (). The optimum extraction conditions were 11.8 mL·g−1 of ethanol/bran ratio, 60 min, 65°C, and 7 extraction cycles. The experimental yield was 35.52% which is close to the value predicted by the BBD model (34.36%). By applying the two combinations of factors generated by the path of steepest descent, the first combination (12 mL/g, 60 min, 65°C, and 8 cycles) allowed a yield of 35.50%, while the second (11 mL/g, 70 min, 55°C, and 8 cycles) exhibited a yield of 39.90% which is higher than that from the BBD model (). Compared to the first combination generated by the path of steepest descent, the BBD model conditions were economical with small number of cycles and low ethanol/bran ratio.