Formulation Optimization and Evaluation of Probiotic Lactobacillus sporogenes-Loaded Sodium Alginate with Carboxymethyl Cellulose Mucoadhesive Beads Using Design Expert Software

Table 1

Experimental plan and observed response values from randomized run-in factorial design layout.

Formulation batch code	Independent variable				% entrapment efficiency ()	Viability in SGF over 2 hr (log CFU/g) ()	% mucoadhesion over 8 hr ()
	Sodium alginate ()		Sodium CMC ()
	Coded value	Actual value	Coded value	Actual value
D1	−1	2.5	−1	0.5	90.88 ± 1.4	8.48 ± 0.3	54 ± 0.81
D2	0	3	−1	0.5	92.33 ± 1.2	8.58 ± 0.4	56.35 ± 0.57
D3	1	3.5	−1	0.5	94.81 ± 1.2	8.45 ± 0.2	58.85 ± 0.57
D4	−1	2.5	0	0.75	91.57 ± 1.6	8.42 ± 0.3	61.75 ± 1.15
D5	0	3	0	0.75	93.39 ± 1.3	8.56 ± 0.2	62.95 ± 0.57
D6	1	3.5	0	0.75	95.44 ± 1.2	8.26 ± 0.2	65.65 ± 0.57
D7	−1	2.5	1	1	92.66 ± 1.2	8.85 ± 0.2	68.85 ± 0.58
D8	0	3	1	1	93.74 ± 2.0	9.34 ± 0.2	73.75 ± 0.57
D9	1	3.5	1	1	95.55 ± 1.8	9.28 ± 0.22	76 ± 1.00