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Journal of Food Quality
Volume 2017 (2017), Article ID 5239487, 8 pages
https://doi.org/10.1155/2017/5239487
Research Article

Development of Single-Walled Carbon Nanotube-Based Biosensor for the Detection of Staphylococcus aureus

1Department of Plant and Food Sciences, Sangmyung University, 31 Sangmyungdae-gil, Dongnam-gu, Cheonan, Chungnam 31066, Republic of Korea
2School of Food Science and Biotechnology, Kyungpook National University, 80 Daehak-Ro, Buk-Gu, Daegu 41566, Republic of Korea

Correspondence should be addressed to Mi-Kyung Park; rk.ca.unk@kimkrap and Jun-Hyun Oh; rk.ca.ums@honuyhnuj

Received 16 March 2017; Revised 16 September 2017; Accepted 8 October 2017; Published 23 November 2017

Academic Editor: Jorge Barros-Velázquez

Copyright © 2017 Hyun-Kyung Choi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The goal of this research is to develop a single-walled carbon nanotube- (SWCNT-) based biosensor to detect Staphylococcus aureus. The specificity of 11 bacteria and polyclonal anti-Staphylococcus aureus antibodies (pAbs) was determined using an indirect ELISA. The pAbs were immobilized onto sensor platform after the hybridization of 1-pyrenebutanoic acid succinimidyl ester (PBASE). The resistance difference () was calculated using a potentiostat. The bacteria detected by the biosensor were observed using a scanning electron microscope (SEM). The optimum concentration of SWCNTs on the platform was determined to be 0.1 mg/mL. The binding of pAbs with S. aureus resulted in a significant increase in resistance value of the biosensor (). The SEM images confirmed the specific binding of S. aureus on the biosensor. The SWCNT-based biosensor was able to detect S. aureus with a limit of detection (LOD) of 4 .