Development of a Chemically Defined Medium for Better Yield and Purification of Enterocin Y31 from Enterococcus faecium Y31

Table 1

Composition of CDM and SDM medium.

Constituent	Content
	CDM	SDM
Amino acids (g L^−1)
L-Asparagine	1.00	1.00
L-Aspartic acid	1.00	—
L-Glutamic acid	1.00	1.00
L -Glutamine	1.00	—
L-Cys	1.00	1.00
L-Alanine	1.00	—
L-Arginine	1.00	1.00
Glycine	1.00	1.00
L-Histidine	1.00	1.00
L-Isoleucine	1.00	1.00
L-Leucine	1.00	1.00
L-Lysine	1.00	1.00
L-Methionine	1.00	1.00
L-Phenylalanine	1.00	1.00
L-Proline	1.00	—
L-Serine	1.00	1.00
L-Threonine	1.00	1.00
L-Tryptophan	1.00	1.00
L-Tyrosine	1.00	—
L-Valine	1.00	—
Nucleic acid bases (g L^−1)
Ademome.SO4	0.02	0.02
Guanine	0.02	—
Inosine	0.02	—
Xanthine	0.02	—
Uracil	0.02	0.02
Thymine	0.02	—
Orotic acid	0.02	—
B vitamins (mgL^−1)
Biotin	20.00	20.00
Para-aminobenzoic acid	10.00	—
Nicotinic acid	2.00	2.00
Ca-pantothenic acid	20.00	20.00
Riboflavin	2.00	2.00
Folic acid	2.00	2.00
Cyanocobalamin	2.00	—
Thiamine	10.00	—
Pyridoxal	20.00	—
Minerals (gL^−1)
Manganese sulfate	0.05	0.05
Magnesium sulfate	0.1	0.10
Ferrous sulfate.7H2O	0.02	—
CaCl2	—	—
Sodium citrate	5.00	5.00
Potassium dihydrogen phosphate	2.00	2.00
Dipotassium hydrogen phosphate	7.00	7.00
Carbon source (gL^−1)
Glucose	15.00	15.00
Others (gL^−1)
Tween 80	1.00	1.00
Glycerol	—	—
NaCl	—	5.00