Development of a Chemically Defined Medium for Better Yield and Purification of Enterocin Y31 from Enterococcus faecium Y31

Table 3

Effect of omitting a single amino acid on the growth of Enterococcus faecium Y31 and Enterocin Y31 production.

Omitted amino acid	ODmax (16 h)	Relative growth rate	Enterocin Y31 production (AU/mg protein)
None (CDM)		100
L-Ala		90
L-Arg		n.g.	—
L-Asp		93
L-Asn		60
L-Cys		44	—
L-Glu		72
L-Gln		84
L-Gly		n.g.	—
L-His		n.g.	—
L-Iso		79
L-Leu		n.g.	—
L-Lys		80
L-Met		n.g.	—
L-Phe		n.g.	—
L-Pro		71
L-Ser		n.g.	—
L-Thr		n.g.	—
L-Try		80
L-Tyr		100
L-Val		98

Note: “n.g.” represents that the strain did not grow and “—” represents that there was no inhibition activity. CDM is explained in Table 1, and means in the same row not sharing a common superscript letter are significantly different from each other ().