Research Article

Nutritional Value and Potential Chemical Food Safety Hazards of Selected Traditional and Conventional Pork Hams from Poland

Table 1

Methods used to determine selected parameters.

ParameterResearch method

ProteinKjeldahl’s method with the use of Büchi wet digester B-426 coupled with scrubber B-414 and distillation unit K-314 [6]

WaterOven-drying method [7]

FatSoxhlet method with the use of Büchi extraction system B-811 [8]

Sodium chlorideMohr’s method [9]

Total ashIncineration in °C with prior carbonization with the use of Nabertherm P-300 furnace [10]

Nitrates and nitritesEnzymatic reduction of nitrates to nitrites and with the use of Thermo Scientific Aquamate Plus UV-Vis Spectrophotometer [11]

Total phosphorus expressed as P2O5With the use of Thermo Scientific Aquamate Plus UV-Vis Spectrophotometer [12]

Added phosphorus expressed as P2O5Calculated according to the equation

(where AD is the added phosphorus, TP is the total phosphorus, and is protein)

Water/protein ratios (Feder’s numbers)Calculated according to the equation

(where FN is Feder’s number, is water, and is protein)

Ca, Fe, Mg, K, ZnAtomization in the flame with the deuterium lamp background correction Samples of 0.45 to 0.55 g were mineralized in a mixture of 65% HNO3 and 30% H2O2 (7 : 1 V/V) at 200°C in a Milestone Start D microwave. The obtained solutions were quantitatively transferred to volumetric flasks and diluted to 50 ml. The solutions were analyzed by atomic absorption with the use of Thermo Scientific iCE 3500 apparatus

Cd, Cr, Cu, PbAtomization in a graphite tube, with the background correction based on the Zeeman effect. Samples of 0.45 to 0.55 g were mineralized in a mixture of 65% HNO3 and 30% H2O2 (7 : 1 V/V) at 200°C in a Milestone Start D microwave. The obtained solutions were quantitatively transferred to volumetric flasks and diluted to 50 ml. The solutions were analyzed by atomic absorption with the use of Thermo Scientific iCE 3500 apparatus