Incineration in °C with prior carbonization with the use of Nabertherm P-300 furnace [10]
Nitrates and nitrites
Enzymatic reduction of nitrates to nitrites and with the use of Thermo Scientific Aquamate Plus UV-Vis Spectrophotometer [11]
Total phosphorus expressed as P2O5
With the use of Thermo Scientific Aquamate Plus UV-Vis Spectrophotometer [12]
Added phosphorus expressed as P2O5
Calculated according to the equation
(where AD is the added phosphorus, TP is the total phosphorus, and is protein)
Water/protein ratios (Feder’s numbers)
Calculated according to the equation
(where FN is Feder’s number, is water, and is protein)
Ca, Fe, Mg, K, Zn
Atomization in the flame with the deuterium lamp background correction Samples of 0.45 to 0.55 g were mineralized in a mixture of 65% HNO3 and 30% H2O2 (7 : 1 V/V) at 200°C in a Milestone Start D microwave. The obtained solutions were quantitatively transferred to volumetric flasks and diluted to 50 ml. The solutions were analyzed by atomic absorption with the use of Thermo Scientific iCE 3500 apparatus
Cd, Cr, Cu, Pb
Atomization in a graphite tube, with the background correction based on the Zeeman effect. Samples of 0.45 to 0.55 g were mineralized in a mixture of 65% HNO3 and 30% H2O2 (7 : 1 V/V) at 200°C in a Milestone Start D microwave. The obtained solutions were quantitatively transferred to volumetric flasks and diluted to 50 ml. The solutions were analyzed by atomic absorption with the use of Thermo Scientific iCE 3500 apparatus