Phytochemical Characterization, In Vitro Antioxidant Activity, and Quantitative Analysis by Micellar Electrokinetic Chromatography of Hawthorn (Crataegus pubescens) Fruit

Figure 1

Electropherogram 217 nm (a) and HPLC 230 nm chromatogram (b) of a phenolic extract of Crataegus pubescens fruits. The spectral pattern and identity of the major compounds are shown in (c); 1. (−)-epicatechin; 2. (+)-catechin; 3. procyanidin dimer; 4. procyanidin trimer; 5. procyanidin tetramer; 6. chlorogenic acid. Calibration curves were linear for both methods for concentration between 10 and 160 µM for (−)-epicatechin and (+)-catechin, with correlation coefficients of 0.9901 and 0.9959, respectively, and in a range of 10–240 µM with a correlation coefficient of 0.9976 for chlorogenic acid. The electropherogram with the resolved standards is presented (inset) in (a).

(a)

(b)

(c)