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Journal of Food Quality
Volume 2018, Article ID 8478471, 9 pages
https://doi.org/10.1155/2018/8478471
Research Article

Methods for Extraction of Muscle Proteins from Meat and Fish Using Denaturing and Nondenaturing Solutions

Department of Agricultural Food and Environmental Sciences, University of Foggia, Via Napoli, 25-71121 Foggia, Italy

Correspondence should be addressed to Rosaria Marino; ti.gfinu@oniram.airasor

Received 28 September 2018; Revised 7 November 2018; Accepted 12 November 2018; Published 28 November 2018

Guest Editor: José M. Díaz-Cruz

Copyright © 2018 Antonella della Malva et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The objective of the present study was to test two extraction methods including solutions with different ionic strengths on the extraction and separation of the myofibrillar proteins from meat and fish muscles of different species. Meat samples from longissimus thoracis muscle of beef and lamb, pectoralis major muscle of chicken, and dorsal white muscle of fish from sole (Solea solea), European hake (Merluccius merluccius), and sea bass (Dicentrarchus labrax) were analyzed. The extraction method using nondenaturing solution led to a major extraction of high molecular-weight proteins as myosin heavy chain, α-actinin, and desmin; on the contrary, the denaturing method provided a good protein extractability of proteins and fragments with low molecular-weight as actin, troponin-T, tropomyosin, and myosin light chain 1 and 2 proteins for the most meat and fish samples. The nondenaturing extraction method showed several advantages resulting in time and labour saving and in minimizing the use of toxic and polluting agents.