Phenolic Analysis for Classification of Mulberry (Morus spp.) Leaves according to Cultivar and Leaf Age

Table 1

Identification of phenolic compounds of mulberry leaves and the data taken from HPLC-DAD and HPLC-MS.

Peak	RT (min)	λmax (nm)	Mass spectra				Tentative compound
			Positive ion (m/z)		Negative ion (m/z)
			[M + H]^+	Fragment ions	[M − H]^−	Fragment ions
Caffeoylquinic acid isomers
1	13.1	239, 325	355	163, 135	353	191, 179	Caffeoylquinic acid isomer I^2
2	16.5	241, 325	355	163, 135	353	191	5-O-Caffeoylquinic acid^1
3	17.4	240, 325	355	163, 135	353	191, 179, 173	4-O-Caffeoylquinic acid^1
5	19.5	237, 317	355	163, 135	353	191	Caffeoylquinic acid isomer II^2
Flavonol derivatives
4	18.2	265, 347	757	611, 287, 449	755	609	Kaempferol-hexoside derivative^2
6	21.4	256, 354	757	303, 611, 627, 465	755	625	Quercetin-rutinoside derivative^2
7	23.7	265, 354	741	595, 287, 449	739	—	Kaempferol-rutinoside derivative^2
8	25.6	256, 354	611	465, 303	609	—	Quercetin-3-O-rutinoside^1
9	27.1	256, 353	465	303	463	—	Quercetin-hexoside^2
10	29.1	265, 347	595	449, 287	593	—	Kaempferol-3-O-rutinoside^1
11	29.6	256, 354	551	303	549	505	Quercetin-(malonyl)-hexoside^2
12	31.0	265, 347	449	287	447	—	Kaempferol-hexoside^2
13	34.3	265, 347	535	287	533	489	Kaempferol-(malonyl)-hexoside^2

Identification of phenolic compound. ¹According to UV spectrum and MS data in combination with retention time of the reference standard. ²According to UV spectral and MS data comparison with the data obtained from previous studies.