Preliminary In Vitro Evaluation of the Probiotic Potential of the Bacteriocinogenic Strain Enterococcus lactis PMD74 Isolated from Ezine Cheese
Table 1
Indicator strains and the antimicrobial activity spectrum of E. lactis PMD74.
Strains
Sourcea
Growth conditionsb
Antimicrobial activityc
Agar spot test
Well diffusion
Lactobacillus plantarum LMG2003
NLH
MRS, 37°C
+++
NZ
Lactobacillus sake NCDO2714
NLH
MRS, 37°C
+++
+++
Lactococcus lactis SIK83
NLH
M17, 30°C
NZ
NZ
Pediococcus pentosaceus FBB611
NLH
MRS, 37°C
+++
++
Pediococcus pentosaceus BH105
AUFF
MRS, 37°C
NZ
NZ
Bifidobacterium longum CHL28
NLH
MRS, 37°C
++
NZ
Bacillus cereus FM1
NLH
LB, 37°C
NZ
NZ
Listeria monocytogenes ATCC 7644
AUFF
LB, 37°C
+++
+++
Staphylococcus aureus ATCC 6538
AUFF
LB, 37°C
+++
++
Salmonella enterica serotype Typhimurium SL1344
AUFF
LB, 37°C
+++
NZ
Micrococcus luteus NCIMB8166
AUFF
LB, 37°C
NZ
NZ
Enterococcus faecalis LMG2708
NLH
BHI, 37°C
+++
++
Enterococcus faecalis ATCC 29212
AUFF
BHI, 37°C
+++
++
Escherichia coli ATCC 26922
AUFF
LB, 37°C
+++
++
Escherichia coli LMG3083 ETEC
NLH
LB, 37°C
+++
++
aNLH: Agricultural University of Norway; AUFF: Faculty of Science, University of Ankara, Tukey. bMRS: de Man Rogosa Sharpe (Merck, Germany); BHI: brain heart infusion (Merck, Germany); LB: Luria Bertani (Merck, Germany); M17 (Merck, Germany). cNZ: no inhibition zone (diameters of inhibition zones are +: 1–5 mm, ++: 6–10 mm, and +++: ≥11 mm).