Influence of Heat Processing on DNA Degradation and PCR-Based Detection of Wild-Type and Transgenic Maize

Figure 4

PCR analysis of exogenous 258 bp amplicon from the samples treated at high temperature: (a) 100°C and (b–d) 121°C. Exposure duration: lanes 1 and 5 = 0 min; lanes 2 and 6 = 60 min; lanes 3 and 7 = 180 min; lanes 4 and 8 = 300 min. Extraction was performed by the CTAB method (a, b) and DNeasy kit (c, d). Samples: wild-type maize (lanes 1–4 in (a–d)), event Bt176 (lanes 5–8 in (a–c)), event MON810 (lanes 5–8 in (d)), water (lane 9 in (a–d)), and GelPilot 100 bp ladder (Qiagen) (lane 10 in (a–d)).

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(b)

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