Immobilization of Alkaline Collagenase from Bacillus subtilis onto Sulfonated Polystyrene Nanospheres for Hydrolysis of Tilapia Collagen

Table 1

Factors and levels of the orthogonal test.

Level	A	B	C
	Volume ratio of the immobilization carrier to the enzyme (mL : mL)	Immobilization temperature (°C)	pH of the immobilized system
1	3 : 50	20	3.5
2	4 : 50	25	4.0
3	5 : 50	30	4.5