Review Article

Effects of Nonthermal Plasma on Wheat Grains and Products

Table 1

Experimental conditions and results of some attempts to influence wheat grains and flour properties.

Discharge descriptionExposure timeResultsPaper

DBD, atmospheric pressure, air, 80 kV, 50 Hz20 minBacteria and fungi reduction of 2.5 log10[36]
Grain surface hydrophobicity decreased
Minimal or negative influence on germination

DBD, atmospheric pressure, air, AC 20 kV, 14 kHz0–120 s1 log10 reduction of natural bacteria and 2 log10 reduction of filamentous fungi[37]
Grain water uptake increased
Germination increased, optimum at 30 s, over 70 s lower than control
Increase in dry weight and vigor index, optimum for 30 s

DBDSignificant reduction of microbial contamination[38]
No or negative (over 20 min) influence on germination

DBD, atmospheric pressure, air, AC 10 kV, ca 13 kHz20 sTotal reduction of larvae of flour beetles Tribolium confusum and Ephestia kuehniella[41]
No significant changes in fat, protein, ash, and moisture content of flour

DBD, atmospheric pressure, air, 17 kV, 50 Hz4 minImprovement of water uptake of grain surface[44]
Germination potential, germination index, germination rate, and vigor index increased
Shoot length, root length, dry weight, and fresh weight increased
Penetrating of active species into grains improves soluble protein content and α-amylase activity

DBD, atmospheric pressure, air, AC 13 kV, 50 Hz13 minEtching effect on the grain surface; improvement of water uptake[45]
Germination potential, germination rate, germination index, and vigor index increased
Root length, shoot length, fresh weight, and dry weight of the seedlings increased, optimum at 7 min
Enhanced the osmotic-adjustment products and proline and soluble sugar contents

DBD, atmospheric pressure, air, AC 15 kV in amplitude, 50 Hz5–30 minGrain wettability increased[47]
Minimal influence on germination
Longer roots and sprouts and heavier roots increase of the R/S ratio

DBD, atmospheric pressure, air, AC 18 kV amplitude, 50 Hz5–45 minImproved the germination rate, speed of germination, and speed of growing in the early stage; over 30 min properties decreased under untreated[61]
Improved length of roots and sprout, vigor index, number of roots; over 30 min decreased under control

DBD, low pressure 10 torr, Ar/O2 and Ar/Air, 5–10 kV, 3–8 kHz90 sImproved germination rate and seedling vigor[42]
Decrease of root length and root dry weight; increase of shoot length and shoot dry weight
Increase of soluble proteins in both roots and shoots

DBD atmospheric pressure, air, 10 kV, 6 kHz and up to 24 kV, 50–Hz5–35 minReduction of lipase and lipoxygenase enzymes activity; enhance the shelf life of grains[63]
DBD, variable gas or air, 0.5–3 kV, 50 Hz, from low pressure under 1 mbar to atmospheric pressure3 min100% mortality of Tribolium castaneum beetle[66]
No significant changes in color of flour

DBD atmospheric pressure, air, 60–70 kV5 and 10 minElastic and viscous moduli of dough from strong wheat flour increased; no variation in the dissipation factor tan δ; improvement of the dough strength[2]

DBD at atmospheric pressure, 10 kV at 50 Hz3 minReduced development of Rhizopus nigricans fungal disease[39]

Cleaning of the seeds surface
DBD at 0–50 kV and 50 Hz in the Ar, N2, air, or O2 atmosphere1–19 minGermination potential increased[59]
Etching effects on the seed coat
Shoot and root length increased
Increase of soluble protein production

DBD in air, 80 kV, 50 Hz30, 60, or 180 sGermination rate enhanced[36]
Positive effects on seedling growth
Changes of seed surface in seed pH, nitrites, nitrates, and malondialdehyde content

Not specified, probably DBD, atmospheric air120 sNo change in the total count of aerobic bacteria and mould in flour[67]
Total free fatty acids and phospholipids reduced; some oxidation markers increased
Treated flour did produce a stronger dough

DBD at 6–10 kV, 5–15 kHz in the Ar at atmospheric pressure0–60 minInactivation of bacterial G. stearothermophilus endospores[40]

Low-pressure plasma15 sSpike length and number of grains per ear improved; grain weight increased by 1%[65]

Low pressure 140 Pa·MW discharge3–40 minLongitudinal cracks and fast wetting of grain surface[43]
Germination enhanced
Shoot phenolic compounds increased

Plasma discharge at frequency 3109 MHz, power 60 W, 80 W, and 100 W, helium atmosphere15 sImproving seed germination potential and germination rate[58]
Improving plant height, root length, and fresh weight
Higher chlorophyll content, nitrogen, and moisture content

CD, atmospheric pressure, air, AC 8 kV, 0.1–83 kHz10 sOne-order decrease of fungal colonies[35]
No significant differences on lengths of the root and shoot and on weight of the seedling
No significant differences in dry matter of plants’ root length decreased to cca one-half

Gliding arc discharge, atmospheric pressure, H2O/air, H2O/O2, H2O/O2/air, 5 kV3–15 minGrain surfaces more rough; enhanced water permeability into the grains[48]
Positive (till 6 min) and negative (over 6 min) influence on germination and plant length
Increase in total number of grain per spike, grain weight, and yield

Glow discharge, 1–6 kV, 3–5 kHz, low pressure 1.3 kPa, air, and air + O23–15 minEnhancement of water absorption property; increased germination rate[46]
Increase of dry weight, spike length, number of spikelet, and number of grains
Yield increased by 20% from plants

RF 13.56 MHz, air or air + He mixture, low pressure 30–200 Pa90 sGermination increased[60]
Lengths of root and shoot and dry weight increased

Plasma-activated water produced by a transient spark dischargeActivation time 1–40 minImproved germination and development of the seedlings[33]
Improved content of photosynthetic pigments in the leaves and soluble protein in the roots
Suppressed activity of antioxidant enzymes