Quantitative Detection of Beef and Beef Meat Products Adulteration by the Addition of Duck Meat Using Micro Drop Digital Polymerase Chain Reaction

Table 2

Detection by digital PCR of mixed samples with known adulteration ratios.

Number	Beef mass(mg)	DNA copy number (copies/μL)			Average value(copies/μL)	Coefficient of variation(%)	Measured cassava mass (mg)	Relative error(%)
1	5.0	134	129	142	135 ± 6.55	4.86	5.97	19.4
2	10.0	225	220	230	225 ± 5.00	2.22	11.20	12
3	15.0	339	302	289	310 ± 25.94	6.62	16.12	7.46
4	20.0	366	354	369	363 ± 7.94	2.19	19.25	−3.75
5	25.0	485	492	493	490 ± 4.36	0.89	26.60	6.4
6	30.0	535	554	540	543 ± 9.85	1.81	29.69	−1.03
7	35.0	620	618	628	622 ± 5.29	0.85	34.22	−2.22
8	40.0	715	720	710	715 ± 5.00	0.70	39.61	−0.98
9	45.0	799	800	815	805 ± 8.96	1.11	44.83	−0.38

Note. Average values are provided as mean ± standard deviation.