Homogenate Extraction of Polysaccharides from Pine Nut Meal: Optimization and Comparison with Other Extraction Methods by Characterizing Their Extracts

Zhang, Hongwei; Zhang, Yi; Bao, Yihong; Yu, Dianyu; Jiang, Lianzhou

doi:https://doi.org/10.1155/2020/7918703

Journal of Food Quality

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Abstract Introduction Materials and Methods Results and Discussion Conclusions Data Availability Conflicts of Interest Acknowledgments Supplementary Materials References Copyright Related Articles

Research Article | Open Access

Volume 2020 | Article ID 7918703 | https://doi.org/10.1155/2020/7918703

Homogenate Extraction of Polysaccharides from Pine Nut Meal: Optimization and Comparison with Other Extraction Methods by Characterizing Their Extracts

Hongwei Zhang,^1,2Yi Zhang,³Yihong Bao,¹Dianyu Yu,²and Lianzhou Jiang²

Academic Editor: Barbara Speranza

Received27 Jan 2020

Revised26 Feb 2020

Accepted11 Mar 2020

Published03 Jun 2020

Abstract

Homogenate extraction technology was applied to extract polysaccharides from pine nut (Pinus koraiensis) meal, a food waste from its oil processing. The first objective was to study the effects of liquid-solid ratio, homogenate extraction time, and temperature on the yield of pine nut meal polysaccharides (PNMP) using single-factor and Box–Behnken design. The optimal processing conditions were liquid-solid ratio of 43.23 mL/g at 35°C for 2 min 50 sec that produced PNMP yield of 11.7%. Extraction factors interacted with each other significantly (). The second objective was to compare the homogenate extract with those produced using other methods including ultrasonic, microwave, and hot water extraction. Polysaccharides were separated using HPLC based on molecular weight (MW) and characterized with the polysaccharide and protein contents of each fraction. The homogenate PNMP had the most of small size (<100 kDa) polysaccharides, and its glycoprotein fractions generally contained higher level of protein, as well as a high ABTS⁺ scavenging activity observed by HPLC-UV-ABTS⁺ assay. FTIR analysis was used to characterize the functional groups. Homogenate extraction is superior in producing PNMP compared with other common methods, and its PNMP extract has potential antioxidant benefit.

1. Introduction

Pinus koraiensis is a plant distributed in eastern Asia such as Korea and China; the produced pine nut, a major commercial nut products, is rich in nutrients including oils, fatty acids, proteins, polysaccharides, vitamins, and minerals with various beneficial effects [1–3]. Most research on pine nut aim to discover its oils, and thus the defatted pine nut meal (PNM) has been commonly considered as a by-product or agri-food waste from pine nut oil production. To add edible value into PNM, there have been a few research studies on the development of proteins and peptides from PNM, including novel peptides identified from PNM with antioxidant capacity and immunity enhancement ability [4, 5]. Plant polysaccharides and oligosaccharides are natural constituents that can benefit human health in terms of antioxidant ability, anti-inflammation, immune-regulation, and hyperglycemia management [6, 7]. Thus, PNM is used in this study to develop a new strategy for high value utilization of PNM.

Polysaccharides is a major component in pine nut. Recently, a polysaccharide complex with molecular weight (MW) of 23 kDa containing carbohydrate, protein, and uronic acid, was separated from pine nut; its hepatoprotective effects against a few types of liver injury were also investigated in vitro [8]. Another 20.6 kDa polysaccharides composed of arabinose, rhamnose, and glucose were purified from pine nut with various health benefits [9]. However, there have been little studies reported on producing crude polysaccharides from pine nut meal by different extraction methods.

Commonly used extraction methods have many limitations in food industry, such as low yield, flavor and nutrients loss, energy consumption, and low efficiency. Homogenate extraction is to extract constituents from food materials in liquid via a combination of high-speed mechanical shearing, mixing, cutting, and smashing [10]. Homogenate extraction method can be applied at low temperatures for a short time when it is compared with other extraction methods; so, it is excellent in food industry to improve food quality, processing efficiency, consumption cost, and manufacturing operation. There have been several studies on the utilization of homogenate extraction for extracting bioactive food compounds such as alkaloids, pigments, and polysaccharides [10–13]. However, there is no report on polysaccharides extracted from pine nut meal using homogenate extraction method. Thus, one of the objectives in this study is to investigate the effects of homogenate extraction conditions such as liquid-solid ratio, extraction time, and temperature on the yield of pine nut meal polysaccharides (PNMP), followed by optimization study using response surface methodology (RSM).

The bioactivity of polysaccharides depend on several factors such as molecular weight, structure, protein level, type of linking, and sulfation degree [14]. In this study, chemical properties were determined including the total polysaccharide content, total protein content, and molecular weight of each polysaccharide fraction, in comparison with the bioactivity of PNMP extracts produced by other commonly-used extraction methods, that is, hot water extraction, microwave extraction, and ultrasonic extraction. Free radical ABTS⁺ was used in a HPLC-UV-ABTS⁺ assay system to characterize the potentials of PNMP extracts acting as natural antioxidants.

2. Materials and Methods

2.1. Pretreatment of Pine Nut Meal

Pine (Pinus koraiensis) nut meal was obtained from Hongtai pinecone Co., Ltd. (Heilongjiang, China). To get refined and defatted pine nut powder, the pine nut meal was degreased with petroleum ether, dried at 50°C, and subsequently sieved through 60-mesh screen.

2.2. Chemicals

Ethanol (food grade) was purchased from GuangFu Group Co., Ltd. (China). All other chemicals and reagents used were of analytical grade. NaCl, KCl, Na₂HPO₄, NaH₂PO₄, KH₂PO₄, MnO₂, KBr, H₂SO₄, and ABTS (2,2′-Azino-bis-(3-ethylbenzthiazoline-6-sulphonate) were purchased from sinopharm Chemical reagent Co., Ltd. (Shanghai, China). HPLC grade water was obtained from a MilliQ water purification system (Millipore, Bedford, New Hampshire, USA).

2.3. Extraction of Pine Nut Meal Polysaccharides (PNMP) Using Different Methods

2.3.1. Ultrasonic Extraction

The pretreated pine nut powder (2.5 g) was soaked with distilled H₂O (100 mL) in a 250 mL flat bottom flask placed in the ultrasonic extraction device (XH-2008D, Beijing Xianghu Science and Technology Co., Ltd., Beijing, China) with power of 800 w at 90°C for 30 min and a sequential extraction conducted at the same condition. After extraction, the mixture was centrifuged at 3000 for 10 min, and the supernatant was collected. The supernatant was first concentrated using rotary evaporation at 50°C and then precipitated by adding dehydrated ethanol to a final concentration of 80% (v/v) and placed at 4°C for 24 h. The mixture was centrifuged at 3000 for 20 min, and the sediment was collected and freeze-dried as crude PNMP powder. The crude PNMP yield (%) was calculated using the following equation:

2.3.2. Microwave Extraction

The pretreated pine nut powder (2.5 g) was soaked with distilled H₂O (100 mL) in a 250 mL flat bottom flask placed in the microwave extraction device (XH-100A, Beijing Xianghu Science and Technology Co., Ltd., Beijing, China) with power of 800 w at 90°C for 3 min and a sequential extraction conducted at the same condition. The process after extraction was the same as Section 2.3.1.

2.3.3. Hot Water Extraction

The pretreated pine nut powder (2.5 g) was soaked with distilled H₂O (100 mL) in a 250 mL flat bottom flask placed in the water bath at 90°C for 60 min and a sequential extraction conducted at the same condition. The process after extraction was the same as Section2.3.1.

2.3.4. Homogenate Extraction

The pretreated pine nut powder (2.5 g) was soaked with distilled H₂O (100 mL) in a 200 mL extraction tank using high-speed homogenizer (Supplementary Figure 1; T18, IKA Co., Ltd., Guangzhou, China) with 20,000 rpm at 30°C for 2 min. The process after extraction was the same as Section 2.3.1.

2.4. Response Surface Methodology (RSM) and Design

A three-factor Box–Behnken design (BBD) at three levels (−1, 0, +1) was carried out to study the homogenate extraction of PNMP. Based on the results of single-factor experiment, the levels of three factors were selected, that is, liquid-solid ratio (X₁, mL/g), extraction time (X₂, min), and extraction temperature (X₃, °C), and the response was PNMP yield (%). The BBD design consisted of 17 experimental runs including 5 center points and 12 factorial points, as shown in Table 1.

2.5. Determination of Total Polysaccharides and Protein

The total polysaccharides content of PNMP was determined by the phenol-sulfuric acid method using _D-glucose as the standard [15]. The protein content of PNMP was measured following the Bradford method using bovine serum albumin as the standard [16].

2.6. HPLC Analysis

A size exclusion chromatography analysis was performed on an Agilent 1100 Series HPLC System (Agilent Technologies, Pittsburg, USA) with G2000PW column (7.5 × 300 mm, 5 μm sized particles) (Tosoh Corporation, Tokyo, Japan). 0.1 M phosphate buffer (pH 6.8) was used mobile phase. The UV detector at 280 nm and the RI detector are used in series. The column and refractive index (RI) detector temperature were at 35°C. Flow rate was 1 mL/min. The injection volume was 100 μL (1 mg/mL).

2.7. Online HPLC–UV–ABTS⁺ Radical Scavenging Activity Analysis

The online HPLC–UV–ABTS⁺ radical scavenging assay was based on HPLC and HPLC postcolumn analyses [17, 18]. Briefly, the ABTS⁺ solution was prepared by adding 2 g MnO₂ to 1 L 0.02 mM ABTS⁺ in PBS buffer (pH 7.4, containing 8 g NaCl, 1.4 g Na₂HPO₄, 0.15 g KCl, and 0.27 g KH₂PO₄). The solution was gently stirred for 10 min at room temperature and filtered (0.2 μm, 33 mm, Nylon, PALL). The method of HPLC separation with UV detection was the same as Section 2.6. For the HPLC postcolumn analyses with a 2487 UV detector (Waters Corporation, Milford, MA, USA) to analyze online oxidation, the ABTS⁺ solution was added at a flow rate of 0.8 mL/min, and the detection was performed at 414 nm.

2.8. FTIR (Fourier Transform Infrared) Spectroscopic Analysis

FTIR spectroscopic measurements were conducted using a FTIR spectrometer (IRAffinity-1, Shimadzu Inc., Japan) according to the KBr pressed disc method. The spectrum was obtained between the wave number range of 4000–400 cm⁻¹ with a resolution of 1 cm⁻¹.

2.9. Statistical Analyses

All the experiments were performed with triplicates, and data was analyzed using the general linear model’s procedure for variance (ANOVA) of the Statistix software 8.1 (Analytical Software, Tallahassee, FL, USA). LSD all-pairwise multiple comparisons was performed to identify significant differences () between individual means. The RSM data was analyzed using the Design-Expert software (version 8.0 trial, Stat-Ease, Inc., Minneapolis, USA).

3. Results and Discussion

3.1. Single Factor Experiments on the Homogenate Extraction of PNMP

To study the polysaccharide yield (%) of the PNMP extracts produced using homogenate extraction method, the effects of liquid-solid ratio (mL/g), extraction time (min) and extraction temperature (°C) were investigated at first, as shown in Figure 1.

(a)

(b)

(c)

Regarding the effect of liquid-solid ratio, homogenate extraction was performed at liquid-solid ratio of 10, 20, 30, 40, 50, and 60 mL/g, respectively, at extraction temperature of 20°C for 2 min with shear speed of 20,000 rpm. As shown in Figure 1(a), the polysaccharide yield increased significantly when the liquid-solid ration increased from 10 to 40 mL/g () and obtained the maximum value of 10.40% at 60 mL/g. It can be explained that a liquid-solid ratio >40 mL/g favored the diffusion of extraction liquid into the pine nut meal powder cells for a better dissolution of polysaccharides [19]. Previous reports showed similar trend that the polysaccharides from extracts eventually showed a stable level up to a certain level of liquid-solid ratio [20, 21]. Thus, liquid-solid ratio of 30, 40, and 50 mL/g was used for the subsequent RSM experiment.

Regarding the effect of extraction time, homogenate extraction was performed for 1, 2, 3, 4, and 5 min, respectively, at liquid-solid ratio of 40 mL/g and 20°C with shear speed of 20,000 rpm. As shown in Figure 1(b), there was a dramatically improvement in the polysaccharides yield when extraction time increased from 1 to 2 min () and became stable after 3 min (). Thus, extraction time of 2, 3, and 4 min was chosen for the subsequent RSM experiment.

Regarding the effect of extraction temperature, homogenate extraction was performed at 20, 25, 30, 35, 40, 45, and 50°C, respectively, at liquid-solid ratio of 40 mL/g for 2 min with shear speed of 20,000 rpm. The polysaccharide yields of PNMP gradually enhanced with increasing of temperature from 20 to 35°C (), as shown in Figure 1(c). The yields were stable at 11% when extraction temperature was above 35°C (). Thus, extraction temperature of 30, 35, and 40°C was selected for the subsequent RSM experiment.

3.2. The RSM Model of PNMP Yield by Homogenate Extraction

The PNMP yields of the extracts produced by homogenate extraction method under various conditions designed by BBD are presented in Table 1. The difference in the response value indicated that homogenate extraction factors including liquid-solid ratio (X₁, mL/g), extraction time (X₂, min), and extraction temperature (X₃, °C) influenced the PNMP yield.

Response surface analysis data for the response in Table 2 shows that the quadratic (second-order) polynomial model was significant ( value <0.0001, F value = 52.78). For the fitted model, as shown in equation (2), the values indicated that the linear effect X₁, quadratic effects , and interaction terms X₁X₂, X₁X₃, X₂X₃ were highly significant, and the linear effect X₂ ( value ) was significant. X₃ with value of 0.5796 suggested that the extraction time was not a significant effect. The lack of fit () was not significant indicating that the model fitted the experimental data well. R² and adjusted R² were 0.9855 and 0.9668, respectively, which is desirable.

3.3. The Effects of Homogenate Extraction Conditions on PNMP Yield

The contour plots of the model in Figure 2 show the interaction relationship of the studied extraction factors as a function of PNMP yield. All three contour plots are elliptical in shape, suggesting strong interactions between two extractions factors [22] that could also be indicated from Table 2. It can be seen that the effect of liquid-solid ratio (X₁) has the densest contour lines along its homologous axis, followed by effect of extraction time (X₂), implying that X₁ and X₂ were the two factors that would mostly influence the PNMP yield, which could also be suggested from data in Table 2. As shown in Figure 2(a), an increase of liquid-solid ratio (X₁, from 30 to 45 mL/g) combined with an increase of extraction time (X₂, from 2 to 3 min) caused an increase in PNMP yield. Generally, higher volume of extraction liquid combined with higher temperature to extract polysaccharides favors an easier dissolving and faster diffusion of compounds from pine nut meat powder. However, there might be loss and damage of extracted polysaccharides if the liquid volume and temperature were too high. Figure 2(b) showed the effect of liquid-solid ratio (X₁) and extraction temperature (X₃) on the PNMP yield. The highest PNMP yield was observed at liquid-solid ratio of 43 mL/g at 35°C. Figure 2(c) showed the effect of extraction time (X₂) and extraction temperature (X₃) on the PNMP yield. Excessive high temperature above 35°C and longtime above 3 min negatively influenced the PNMP yield, which might be due to the degradation of polysaccharides.

(a)

(b)

(c)

3.4. Optimum Conditions and Validation

The optimum conditions predicted for the polysaccharides extraction from pine nut meal were as follows: liquid-solid ratio of 43.23 mL/g at extraction temperature of 35°C for an extraction time of 2 min 50 sec. The maximum predicted PNMP yield was 11.75% under the optimal conditions. To validate the prediction, PNMP was extracted using the above conditions and the experimental result was 11.68 ± 0.14%, showing no significant difference from the predicted value (). The optimized PNMP was used for subsequent analysis to compare the homogenate extraction method with other commonly used extraction methods.

3.5. Comparision of Homogenate Extraction Method with Others

To compare the efficacy of novel homogenate extraction method with other commonly used extraction methods such as ultrasonic extraction, microwave extraction, and hot water extraction, several assays were conducted on their polysaccharide extracts using methods described in Subsections 2.5–2.8. The PNMP were produced by other methods as indicated in Sections 2.3.1–2.3.3. The PNMP produced using homogenate extraction method was based on optimum conditions in Subsection 3.4.

3.5.1. Polysaccharides Yield and Total Polysaccharides and Protein Contents

Table 3 shows the polysaccharides yield (%), total polysaccharides content (%), and total protein content of the PNMP extract produced using various methods. The polysaccharides yield from homogenate extraction method was 11.7 ± 0.1%, significantly higher than other methods (). Similarly, homogenate extraction method produced the highest total polysaccharides content (43.6 ± 2.1%) and the lowest total protein content (41.7 ± 1.2%) of PNMP extract, but it was not significantly different from ultrasonic extraction method with corresponding values of 42.9 ± 1.8% and 40.5 ± 1.1%, respectively (). However, in terms of processing efficiency, homogenate extraction conducted for 6 min was much shorter than the ultrasonic extraction for 60 min. The above results show that homogenate extraction method is superior to other extraction methods to produce high-level polysaccharides in the extracts. Based on it, a combined method using ultrasonic and homogenate could be investigated on the PNMP extraction in the future.

3.5.2. HPLC Analyses

The size exclusion chromatographic analyses on a HPLC system equipped with RI and UV 280 nm detection for polysaccharides and protein in PNMP extracts, shown as blue and black dashed lines, respectively, were shown in Figure 3. A previous study has discussed that the soluble polysaccharide-protein complex possibly existed and was responsible for the high stability and emulsifying activity of glutelin in pine nut [23]. The peaks appearing in the elution profile from HPLC analyses suggest that the PNMP extract consisted of glycoproteins. It can be seen that there were three major peaks presented at 5–7 min, 9–10 min, and 11–12 min during the elution for extracts produced by all the four extraction methods; however, the numbers of peaks and the area of each peak varied among them, which suggested that different extraction method contributed to difference in polysaccharides/glycoprotein components as well as the amount of each component in PNMP extract. In general, the overall peaks patterns seemed to be similar for microwaves and hot water extraction methods (Figures 3(b) and 3(c)). PNMP extract from ultrasonic method had more peaks especially in fractions eluted after 12 min (Figure 3(a)). Homogenate extraction method generated larger peaks (Figure 3(d)) compared with other methods at 10 min.

(a)

(b)

(c)

(d)

Each of the peak fractions was collected and measured using phenol-sulfuric acid method and Bradford method described in Section 2.5 to determine the total polysaccharide and protein contents, shown as blue and black columns, respectively, in the bar graph below each elution profile in Figure 3. Microwave extraction method and hot water extraction methods led to low content of polysaccharide/protein in the PNMP extracts (Figures 3(b) and 3(c)), especially the hot water extraction, which had the worst effect in producing PNMP enriched product. Generally, ultrasonic extraction method could generate glycoprotein fractions containing higher portion of polysaccharides (Figure 3(a)); in contrast, homogenate extraction method produced fractions with higher amount of protein (Figure 3(d)). For example, the fraction eluted at 8–9 min from ultrasonic extract contained 35 μg/mL polysaccharides and 17 μg/mL protein (Figure 3(a)), but the corresponding elution fraction from homogenate extract contained 15 μg/mL polysaccharides and 36 μg/mL protein (Figure 2). The reason could be that the long-time high-temperature ultrasonic treatment enhanced the hydrolysis and degradation of the biopolymers [24, 25] in the PNMP extracts to a larger extent than homogenate extraction treatment.

Based on the peaks detected using RI detector and the standard curve, the exact molecular weight (MW) of each peak was determined. Figure 4 shows the MW distribution of polysaccharides in the PNMP extracts produced by various extraction methods. Homogenate extraction method produced the highest percentage of low MW polysaccharides component, that is, >90% of polysaccharides with MW < 100 kDa. It could be caused by the no thermal polymerization on the PNMP caused by homogenate treatment. Moreover, homogenate extraction produced the most of medium MW polysaccharides (20–100 kDa) but the least of low MW polysaccharides (<20 kDa). A similar MW distribution pattern was found for the PNMP extract from ultrasonic method.

3.5.3. HPLC–UV–ABTS⁺ Analyses

An online HPLC–UV–ABTS⁺ system was used, which was detected at UV 414 nm, shown as the red dashed lines in Figure 3, and the corresponding ABTS⁺ scavenging activity of each peak was presented as red columns in the bar graph below each elution profile. Online HPLC–UV–ABTS⁺ based assay is sensitive and relatively simple [17] and has been successfully used in the rapid detection of antioxidants such as flavonoids in azalea flowers [26] and phenolic antioxidants in Salsola komarovii [27]. Regarding the UV 414 nm plots in Figure 3, the larger the negative peak area, the higher th antioxidant activity of the eluted components. In this case, the peak eluted at 6–7 min had the highest ABTS⁺ scavenging activity for ultrasonic, microwave, and hot water extracts (Figures 3(a)–3(c)); however, there was another peak found with higher antioxidant activity for homogenate extract (Figure 3(d)). Thus, the homogenate extraction method produced the most antioxidant PNMP extract, especially the eluted peaks at ∼9 min and ∼10 min with ABTS⁺ scavenging activity of 18% and 32%, respectively, while the conventional hot water extraction produced the least. By combining with the HPLC assay and Figure 4, it can be verified that the large amount of medium MW polysaccharides and glycoprotein components in the PNMP extract produced by homogenate extraction was responsible for its high antioxidant activity.

3.5.4. FTIR Analyses

Figure 5 is the FTIR spectra of the PNMP extracts using various extraction methods. As reported previously, the wide absorption bands at 3430 cm⁻¹ resulted from the hydroxyl stretching vibrations of the polysaccharides or the possibly existing bound water in the PNMP extracts, and the weak absorption bands at 2935 cm⁻¹ were originated from C-H stretching of methyl group. The intense peaks at approximately 1625 cm⁻¹ were attributed to C=O asymmetric stretching vibration in a carbonyl group or the possibly existing bound water in the PNMP extracts, and the absorbance at 1430 cm⁻¹ was associated with the C-H bending or stretching frequencies [28, 29]. Similar FTIR spectra were found for polysaccharides from pine cones [30]. The shape of the amide I band located at 1600–1700 cm⁻¹ is used to determine the secondary structure of protein [31, 32], which could also be observed in Figure 5. The four spectra were in similar shape, which suggested all the extracts contained similar components, that is, polysaccharides/protein or glycoprotein.

4. Conclusions

Homogenate extraction technology is revealed to be an efficient, simple, and fast processing method to produce polysaccharides extract from pine nut meal. This present study provides a suitable approach to add value to the underutilized food waste-pine nut meal. Optimization study suggested the optimal conditions to obtain the maximum polysaccharide yield. For homogenate extraction, the relatively low temperature and short extraction time probably contributed to the good quality of the extracted polysaccharides, in terms of the protein content, molecular weight distribution of polysaccharides, and free radical scavenging activity. In comparison with other commonly used extraction methods such as ultrasonic, microwave, and hot water, homogenate extraction is superior. The polysaccharides extracted from pine nut meal showed potential health beneficial effects.

Data Availability

The data used to support the findings of this study are included within the article.

Conflicts of Interest

The authors declare that they have no conflicts of interest.

Acknowledgments

This research was funded by Heilongjiang Provincial Natural Science Foundation of China (Grant no. ZD2019C002) and the National Key Research and Development Program of China (Grant no. 2018YFD0401101).

Supplementary Materials

Supplementary Figure 1: the draft of the device used for homogenate extraction. (Supplementary Materials)

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Copyright © 2020 Hongwei Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Journal of Food Quality

Homogenate Extraction of Polysaccharides from Pine Nut Meal: Optimization and Comparison with Other Extraction Methods by Characterizing Their Extracts

Abstract

1. Introduction

2. Materials and Methods

2.1. Pretreatment of Pine Nut Meal

2.2. Chemicals

2.3. Extraction of Pine Nut Meal Polysaccharides (PNMP) Using Different Methods

2.3.1. Ultrasonic Extraction

2.3.2. Microwave Extraction

2.3.3. Hot Water Extraction

2.3.4. Homogenate Extraction

2.4. Response Surface Methodology (RSM) and Design

2.5. Determination of Total Polysaccharides and Protein

2.6. HPLC Analysis

2.7. Online HPLC–UV–ABTS+ Radical Scavenging Activity Analysis

2.8. FTIR (Fourier Transform Infrared) Spectroscopic Analysis

2.9. Statistical Analyses

3. Results and Discussion

3.1. Single Factor Experiments on the Homogenate Extraction of PNMP

3.2. The RSM Model of PNMP Yield by Homogenate Extraction

3.3. The Effects of Homogenate Extraction Conditions on PNMP Yield

3.4. Optimum Conditions and Validation

3.5. Comparision of Homogenate Extraction Method with Others

3.5.1. Polysaccharides Yield and Total Polysaccharides and Protein Contents

3.5.2. HPLC Analyses

3.5.3. HPLC–UV–ABTS+ Analyses

3.5.4. FTIR Analyses

4. Conclusions

Data Availability

Conflicts of Interest

Acknowledgments

Supplementary Materials

References

Copyright

2.7. Online HPLC–UV–ABTS⁺ Radical Scavenging Activity Analysis

3.5.3. HPLC–UV–ABTS⁺ Analyses