Journal of Food Quality

Journal of Food Quality / 2021 / Article
Special Issue

Applications of Mass Spectrometry in the Analysis of Food Composition and Contaminants

View this Special Issue

Research Article | Open Access

Volume 2021 |Article ID 5597327 |

Lili Cui, Xianzhong Wang, Jie Lu, Jing Tian, Li Wang, Jiaojiao Qu, Zhenhua Liu, Jinfeng Wei, "Rapid Identification of Chemical Constituents in Artemisia argyi Lévi. et Vant by UPLC-Q-Exactive-MS/MS", Journal of Food Quality, vol. 2021, Article ID 5597327, 7 pages, 2021.

Rapid Identification of Chemical Constituents in Artemisia argyi Lévi. et Vant by UPLC-Q-Exactive-MS/MS

Academic Editor: Wei Chen
Received05 Feb 2021
Revised01 Apr 2021
Accepted13 Apr 2021
Published21 Apr 2021


Artemisia argyi Lévi. et Vant is a traditional Chinese medicine with a long history, and its buds and seedlings can be used as vegetables. However, the investigations on the chemical constituents of A. argyi are not sufficient. In this paper, ultra-high performance liquid chromatography tandem hybrid quadrupole-orbitrap mass spectrometry (UPLC-Q-Exactive-MS/MS) was used to identify the chemical constituents of A. argyi. The Q Exactive mass spectrometer was used to collect MS and MS2 data. Finally, 125 compounds were preliminarily identified in A. argyi by comparing the retention time and accurate molecular weight with standard databases such as MZVault, MZCloud, and BGI Library (self-built standard Library by BGI Co., Ltd), including flavonoids, phenylpropanoids, terpenoids, and organic acids.

1. Introduction

Artemisia argyi Lévi. et Vant belongs to Asteraceae family, widely distributed in China. A. argyi is a traditional Chinese medicine with a long history, and its buds and seedlings can be used as vegetables. According to flora of China, A. argyi has functions of warming menstrual cycle, removing dampness, dispersing cold, hemostasis, anti-inflammation, relieving asthma, relieving cough, calming fetus and anti-allergy with whole grass as medicine [1, 2]. According to Chinese Pharmacopoeia (2020), Artemisiae argyi folium (A. argyi leaves) has minor poison, its nature and flavour are warm, pungent, and bitter, and its channel tropism is in liver, spleen, and kidney. A. argyi leaves have warm meridian to stop bleeding, disperse cold, and relieve pain and external clearing damp antipruritic effect [3].

At present, research about chemical constituents of A. argyi was focused on A. argyi leaves. Research showed that the chemical constituents of A. argyi leaves include volatile oil, flavonoids, terpenoids, phenylpropanoids, organic acids, and steroids. Among them, more than 200 volatile oils from A. argyi leaves have been identified, and 182 nonvolatile components have been isolated and identified [2, 4]. Pharmacological studies have shown that A. argyi leaves have many pharmacological effects, such as antibacterial, antiviral, hemostatic, anti-tumor, protecting liver and gallbladder, and anti-oxidation, relieving cough and asthma, analgesia, anti-inflammatory, and immune regulation. Moxibustion has the effect of warming and dispersing cold and treating all diseases [2, 46]. With the development of the health industry, more and more attention has been paid to the study of A. argyi leaves and moxa [6]. In recent years, systematic studies on the chemical constituents of A. argyi leaves or A. argyi have gradually increased [7, 8]. With the mature application of LC-MS/MS technology in the rapid identification of plant and food stuff ingredients [911], research studies on the rapid analysis of nonvolatile components of A. argyi leaves or A. argyi are gradually increasing [1215], but there are few identified compounds. Ultra-high performance liquid chromatography tandem hybrid quadrupole-orbitrap mass spectrometry (UPLC-Q-Exactive-MS/MS) is a new type of liquid chromatography-mass spectrometry developed in recent years, which has the characteristics of high resolution, good quality and precision, and strong qualitative and quantitative ability [16, 17]. Samples can be separated quickly by ultra-high performance liquid chromatography, and accurate molecular weight can be determined by high-resolution mass spectrometry to obtain molecular formula of compounds. Zhumadian region is abundant with A. argyi; however, research about chemical components of A. argyi from Zhumadian region was less. Therefore, in this study, UPLC-Q-Exactive-MS/MS combined with standard substance database was used to rapidly identify the nonvolatile chemical components of A. argyi from Zhumadian region.

2. Materials and Methods

2.1. Instrument

Rotary evaporator (N-1300) was purchased from EYELA. Ultra-performance liquid chromatograph (Waters 2D UPLC) was purchased from Waters, USA. High-resolution mass spectrometer (Q Exactive) was purchased from Thermo Fisher Scientific, USA. Hypersil GOLD aQ column (100 mm × 2.1 mm, 1.9 μm) was purchased from Thermo Fisher Scientific, USA. Low temperature high speed centrifuge (Centrifuge 5430) was purchased from Eppendorf. Vortex finder (QL-901) was purchased from Qilinbeier Instrument Manufacturing Co., Ltd. Pure water meter (Milli-Q) was purchased from Integral Millipore Corporation, USA.

2.2. Reagent

d3-Leucine, 13C9-Phenylalanine, d5-Tryptophan, and 13C3-Progesterone were used as internal standard. Methanol (A454-4) and acetonitrile (A996-4) were both in mass grade, which were purchased from Thermo Fisher Scientific, USA. Ammonium formate (17843–250G) was obtained from Honeywell Fluka, USA. Formic acid (50144–50 mL) was obtained from DIMKA, USA. 95% ethanol (20190320) was obtained from Tianjin Fuyu Fine Chemicals Co., Ltd. Water was supplied by a pure water meter.

2.3. Plants

A. argyi was collected in July 2020 in Wanhei Village, Shangcai County, Zhumadian City, and identified as the aerial part of A. argyi by professor Li Changqin of Henan University. The specimens (No. 2020-08-10) were saved in National Research and Development Center of Edible Fungi Processing Technology, Henan University.

2.4. Preparation of Sample

10 g of A. argyi powder was accurately weighed and impregnated with 25 times 70% ethanol for 2 times at room temperature, each time for 2 days. The extraction was filtered, and filtrate was combined and concentrated. The extract was added with 70% ethanol to 1 g/mL of the original materials and then reserved. 200 μL was sent to BGI Co., Ltd., for chemical composition identification.

2.5. Chromatographic Conditions

Hypersil GOLD aQ column (100 mm × 2.1 mm, 1.9 μm) was used to do LC-MS experiment. The mobile phase was 0.1% formic acid-water (liquid A) and 0.1% formic acid-acetonitrile (liquid B). The following gradients were used for elution: 0–2 min 5% B; 2–22 min 5%–95% B; 22–27 min 95% B; 27.1–30 min 5% B. The flow rate was 0.3 mL/min, the column temperature was 40°C, and the injection volume was 5 μL.

2.6. Mass Spectrometry Conditions

The mass range was 150–1500, the MS resolution was 70000, the AGC was 1e6, and the maximum injection time was 100 ms. According to the strength of the MS ions, TOP3 was selected for fragmentation. The MS2 resolution was 35000, AGC is 2e5, the maximum injection time was 50 ms, and the fragmentation energy were set as 20, 40, and 60 eV. Ion source (ESI) parameter settings: sheath gas flow rate was 40, aux gas flow rate was 10, spray voltage (|KV|) of positive ion mode was 3.80, spray voltage (|KV|) of negative ion mode was 3.20, ion capillary temp was 320°C, and aux gas heater temp was 350°C.

2.7. Data Analysis

UPLC-MS/MS technology was used to systematically analyze the chemical constituents of A. argyi. High-resolution mass spectrometer Q Exactive (Thermo Fisher Scientific, USA) was used to collect data in positive and negative ion modes, respectively, to improve the chemical constituent coverage. Raw mass spectrum data collected by UPLC-MS/MS were imported into Compound Discoverer 3.1 (Thermo Fisher Scientific, USA) for data processing. It mainly includes peak extraction, retention time correction within and between groups, additive ion merging, missing value filling, background peak labeling, and metabolite identification. Finally, the molecular weight, retention time, peak area, and identification results of the compound were derived. The compounds were identified by comparing the retention time, accurate molecular weight, and MS2 data with standard databases such as MZVault, MZCloud, and BGI Library (self-built standard Library by BGI Co., Ltd).

3. Results

3.1. Total Ion Chromatogram

Total ion chromatogram of A. argyi is shown in Figures 1 and 2.

3.2. Identification Results of Chemical Composition

In this study, UPLC-Q-Exactive-MS/MS technology was used to rapidly identify the chemical constituents in A. argyi. The identification of compounds was based on the retention time, MS data, and MS2 data compared with the standard database (MZVault, MZCloud and BGI Library (self-built standard Library by BGI Co., Ltd)). Most flavonoids, phenylpropanoids, and organic acids were easily deprotonated and responded in negative ion mode. Most steroids and terpenoids were easily protonated and responded in positive ion mode. The identification results are shown in Table 1. A total of 125 chemical constituents were identified in A. argyi, including 49 flavonoids, 30 organic acids, 13 phenylpropanoids, 9 terpenoids, 5 amino acids, 2 steroids, 1 phenolic acid, 1 alkaloid, and 15 other compounds.

NumberRetention time (min)Molecular formula[M-H][M+H]+/ [M+NH4]+/ [M+H-H2O]+Compound nameCompound type
Theoretical value (Da)The measured values (m/z)Error (ppm)Theoretical value (Da)The measured values (m/z)Error (ppm)

10.798C4H8N2O3131.04622131.04616−0.44AsparagineAmino acids
20.905C16H18O9353.08778353.08707−1.99Neochlorogenic acidPhenylpropanoids
30.913C7H12O6191.05601191.05557−2.30D-(−)-quinic acidOrganic acids
40.966C4H6O5133.01430133.01425−0.34Dl-malic acidOrganic acids
51.072C6H8O7191.01970191.01927−2.22Citric acidOrganic acids
61.092C9H8O3182.08117182.081391.332-Hydroxycinnamic acidPhenylpropanoids
71.12C4H6O4117.01932117.01909−1.99Succinic acidOrganic acids
81.323C6H10O4145.05062145.05022−2.743-Methylglutaric acidOrganic acids
91.332C5H8O4131.03497131.03461−2.73Glutaric acidOrganic acids
101.41C9H11NO2166.08628166.086430.94L-phenylalanineAmino acids
111.455C9H17NO5218.10339218.10284−2.52Pantothenic acidOrganic acids
123.163C11H9NO2188.07052188.070530.07Indole-3-acrylic acidOrganic acids
134.337C7H12O4159.06628159.06586−2.63Pimelic acidOrganic acids
144.452C16H18O9353.08772353.08716−1.59Cryptochlorogenic acidPhenylpropanoids
164.591C6H12O3131.07130131.07104−1.982-Hydroxycaproic acidOrganic acids
174.63C9H8O4179.03491179.03459−1.80Caffeic acidPhenolic acid
184.839C8H8O3151.04013151.03972−2.702-Hydroxyphenylacetic acidOrganic acids
194.873C8H15NO3172.09792172.09763−1.68N-Acetyl-d-alloisoleucineAmino acids
204.922C16H18O9353.08798353.08734−1.80Chlorogenic acidPhenylpropanoids
214.994C18H28O9387.16599387.16553−1.20{(1r,2r)-2-[(2z)-5-(Hexopyranosyloxy)-2-penten-1-yl]-3-oxocyclopentyl}acetic acidOthers
225.054C19H30O8387.20113387.201140.033-Hydroxy-3,5,5-trimethyl-4-(3-oxo-1-buten-1-ylidene)cyclohexyl β-d-glucopyranosideOthers
235.304C27H30O15593.15126593.1507−0.94Vicenin IIFlavonoids
255.362C25H24O12515.11974515.11871−2.001,3-Dicaffeoylquinic acidPhenylpropanoids
265.46C11H13NO3206.08221206.08176−2.17N-Acetyl-L-phenylalanineAmino acids
275.607C17H20O9367.10343367.10269−2.01(3r,5r)-1,3,5-Trihydroxy-4-{[(2e)-3-(4-hydroxy-3-methoxyphenyl)-2-propenoyl]oxy}cyclohexanecarboxylic acidOthers
285.684C9H8O3163.04006163.03973−2.033-Coumaric acidPhenylpropanoids
305.789C8H14O4173.08194173.08148−2.65Suberic acidOrganic acids
426.456C9H7NO2160.04040160.04007−2.06Indole-2-carboxylic acidOrganic acids
466.516C27H30O14577.15627577.15533−1.63Vitexin rhamnosideFlavonoids
476.546C21H18O12461.07248461.07205−0.93Luteolin 7-glucuronideFlavonoids
496.587C5H9NO2114.05603114.05582−1.85D-ProlineAmino acids
516.685C7H6O3137.02446137.02411−2.553-Hydroxybenzoic acidOrganic acids
556.841C25H24O12515.11944515.11853−1.76517.13405517.134220.344,5-Dicaffeoylquinic acidPhenylpropanoids
587.076C9H16O4187.09763187.09727−1.90Azelaic acidOrganic acids
607.158C15H18O4263.12782263.12778−0.15(3as,10ar,10br)-6,10a-Dimethyl-3-methylene-3,3a,4,5,7,8,10a,10b-octahydrofuro[3′,2':6,7] cyclohepta [1,2-b]pyran-2,9-dioneOthers
617.187C25H24O12515.11931515.11853−1.51Isochlorogenic acid BPhenylpropanoids
627.19C21H18O11447.09180447.091860.14Apigenin 7-O-glucuronideFlavonoids
667.544C15H20O4263.12884263.12845−1.47Abscisic acidOrganic acids
718.522C11H12O4207.06631207.06586−2.15Ethyl caffeatePhenylpropanoids
759.057C12H18O3209.11834209.11795−1.84Jasmonic acidOrganic acids
799.234C15H18O2231.13794231.13792−0.07Atractylenolide ITerpenoids
829.556C18H32O5327.21768327.21722−1.40Corchorifatty acid fOrganic acids
8510.426C12H22O4229.14456229.14415−1.80Dodecanedioic acidOrganic acids
8911.294C15H22O2235.16921235.16913−0.35Artemisinic acidTerpenoids
9211.54C19H18O8373.09297373.09238−1.57Chrysosplenetin BFlavonoids
9912.896C24H30O6415.21151415.2114−0.26Bis (4-ethylbenzylidene) sorbitolOthers
10013.107C20H32O2305.24757305.24741−0.53Arachidonic acidOrganic acids
10113.216C18H34O4313.23846313.238680.70(±)9,10-Dihydroxy-12z-octadecenoic acidOrganic acids
10213.396C15H18O2231.13808231.13791−0.74Dehydrocostus lactoneTerpenoids
10313.636C24H36O2357.27881357.27863−0.51Docosahexaenoic acid ethyl esterOthers
10413.709C18H28O3291.19672291.19680.2912-Oxo phytodienoic acidOrganic acids
10814.59C18H28O3293.21100293.21091−0.329s,13r-12-Oxophytodienoic acidOrganic acids
10914.648C16H30O2255.23177255.23166−0.43Palmitoleic acidOrganic acids
11014.94C18H30O2279.232023279.23166−1.32Α-eleostearic acidOrganic acids
11115.294C14H28O3243.19660243.19652−0.33(r)-3-Hydroxy myristic acidOrganic acids
11215.515C18H30O3295.22663295.226750.419-Oxo-10(e),12(e)-octadecadienoic acidOrganic acids
11316.018C14H28O3243.19657243.19649−0.332-Hydroxymyristic acidOrganic acids
11418.083C16H32O3271.22796271.228030.2516-Hydroxyhexadecanoic acidOrganic acids
11518.536C30H48O3439.35730439.35718−0.27Ursolic acidTerpenoids
11618.586C18H37NO2300.28971300.28955−0.52Palmitoyl ethanolamideOthers
11718.595C20H39NO2326.30542326.30524−0.55Oleoyl ethanolamideOthers
11818.908C18H32O2279.23298279.2330.07Linoleic acidOrganic acids
12120.759C20H34O2307.26319307.26306−0.41Linolenic acid ethyl esterOthers
12220.841C20H41NO2328.32113328.32086−0.81Stearoyl ethanolamideOthers

Note: “—” indicates undetected.

4. Discussion

Eupatilin and jaceosidin as flavonoids and chlorogenic acid as phenylpropanoids were the main components of A. argyi, which were often used as the index components for content determination and quality evaluation [1822], and the content of total flavonoids in A. argyi leaves was as high as 4.48%–11.46% [18]. Flavonoids and phenylpropanoids of A. argyi were also the active ingredients. The flavonoids in A. argyi leaves had anti-tumor, anti-oxidation, anti-platelet aggregation, gastrointestinal smooth muscle protection, and other pharmacological effects. And the phenylpropanoids in A. argyi leaves had pharmacological effects such as antibacterial, anti-inflammatory, antiviral, free radical scavenging, liver protection and gallbladder protection, and lowering blood pressure and blood lipid [12].

Previous studies showed that the chemical components isolated from the A. argyi leaves include flavonoids, terpenoids, phenylpropanoids, organic acids, steroids, etc. [2], and the above chemical components were all contained in A. argyi from Zhumadian, indicating that the chemical components in A. argyi from Zhumadian were relatively diverse with good quality. In this study, in addition to the reported flavonoids, eupafolin, eupatilin, jaceosidin, apigenin, kaempferol, luteolin, hispidulin, isoschaftoside, eriodictyol, naringenin, acacetin, and artemetin isolated from A. argyi leaves, A. argyi from Zhumadian contained 37 other flavonoids such as schaftoside, rutin, isovitexin, and taxifolin. These results revealed that there were abundant flavonoids in A. argyi from Zhumadian. A. argyi from Zhumadian contained most of the reported phenylpropanoids identified from A. argyi [2], such as neochlorogenic acid, cryptochlorogenic acid, chlorogenic acid, isochlorogenic acid B, isochlorogenic acid C, scopoletin, and isofraxidin; besides, A. argyi from Zhumadian also contained other phenylpropanoid compounds, such as 2-hydroxycinnamic acid, esculetin, fraxetin, and 3-coumaric acid. In addition, A. argyi from Zhumadian was rich in terpenoids and organic acids. There were many kinds of active ingredients in A. argyi from Zhumadian, and it was worthy to study systematically to find more natural active ingredients.

Ultra-high performance liquid chromatography (UPLC) has the advantages of high analytical speed, high sensitivity, and solvent saving compared with high-performance liquid chromatography (HPLC) [23]. UPLC is often combined with mass spectrometry for rapid detection of chemical constituents of traditional Chinese medicine. Ultra-high performance liquid chromatography tandem quadruple-time of flight mass spectrometry (UPLC-Q-TOF-MS/MS) and UPLC-Q-Exactive-MS/MS are currently commonly used. Compared to traditional LC-MS/MS, UPLC-Q-Exactive-MS/MS has higher resolution, which can eliminate the interference of sample matrix [17]. Wang et al. [12] investigated the chemical constituents of A. argyi produced in Nanyang by UPLC-Q-TOF-MS/MS technology, and 23 chemical constituents were identified, including 12 flavonoids and flavonoid glycosides, 9 phenylpropionic acids, and 1 coumarin. Li et al. [14] used RRLC-TOFMS technology to rapidly identify the chemical components in A. argyi leaves and identified 31 chemical components. In this study, 125 chemical constituents of A. argyi from Zhumadian were preliminarily identified by UPLC-MS/MS. All of them contain isochlorogenic acid C and eupatilin. The chemical constituents identified in this study were relatively comprehensive, which provides a certain reference for the subsequent studies of A. argyi.

5. Conclusion

In this study, a total of 125 chemical constituents of A. argyi were identified by UPLC-Q-Exactive-MS/MS technology. The UPLC-Q-Exactive-MS/MS technology could be used to quickly and preliminarily identify the chemical constituents of A. argyi, providing a basis for further study on the pharmacological substance basis and resource utilization of A. argyi.

Data Availability

The data used to support the findings of this study are included within the article.

Conflicts of Interest

The authors declare that there are no conflicts of interest regarding this study.


This study was funded by the Basic Project in Science and Technology Agency of Kaifeng City (1908007).


  1. Editorial Committee of flora of China, Flora of China, vol. 76, no. 2, Science press, Beijing, China, 1991.
  2. X. Y. Lang, Y. Zhang, L. B. Zhu et al., “Research progress on chemical constituents from Artemisiae Argyi Folium and their pharmacological activities and quality control,” China Journal of Chinese Materia Medica, vol. 45, no. 17, pp. 4017–4030, 2020. View at: Publisher Site | Google Scholar
  3. National Pharmacopoeia Commission, Pharmacopoeia of the People's Republic of China, vol. 91, China Medical Science and Technology Press, Beijing, China, 2020.
  4. X. L. Zhang and X. W. Chen, “Research progress on chemical constituents and pharmacological activities of Artemisia argyi volatile oil,” Chinese Archives of Traditional Chinese Medicine, pp. 1–19, 2020. View at: Google Scholar
  5. X. L. Zhao and Y. L. Dang, “Advance on chemical constituents and pharmacological effects of Artemisia argyi volatile oils,” Natural Product Research and Development, vol. 31, no. 12, pp. 2182–2188, 2019. View at: Publisher Site | Google Scholar
  6. L. Cao, D. Yu, L. Cui et al., “Research progress on chemical composition, pharmacological effects and product development of Artemisia argyi,” Drug Evaluation Research, vol. 41, no. 5, pp. 918–923, 2018. View at: Google Scholar
  7. S. J. Zhang, Y. L. Ma, J. L. Wang et al., “Chemical constituents of Artemisia argyi,” Chinese Traditional and Herbal Drugs, vol. 50, no. 8, pp. 1906–1914, 2019. View at: Google Scholar
  8. J. L. Wang, Y. L. Ma, D. Wang et al., “Chemical constituents of Artemisia argyi (II),” Chinese Traditional and Herbal Drugs, vol. 51, no. 20, pp. 5114–5122, 2020. View at: Google Scholar
  9. M. Fan, T. Guo, W. Li et al., “Isolation and identification of novel casein-derived bioactive peptides and potential functions in fermented casein with Lactobacillus helveticus,” Food Science and Human Wellness, vol. 8, no. 2, pp. 156–176, 2019. View at: Publisher Site | Google Scholar
  10. J. Ma, S. Fan, L. Sun, L. He, Y. Zhang, and Q. Li, “Rapid analysis of fifteen sulfonamide residues in pork and fish samples by automated on-line solid phase extraction coupled to liquid chromatography-tandem mass spectrometry,” Food Science and Human Wellness, vol. 9, no. 4, pp. 363–369, 2020. View at: Publisher Site | Google Scholar
  11. F. Yang, H. L. Wang, G. Q. Feng et al., “Rapid identification of chemical constituents in Hericium erinaceus based on LC-MS/MS metabolomics,” Journal of Food Quality, vol. 2021, Article ID 5560626, 10 pages, 2021. View at: Publisher Site | Google Scholar
  12. Y. Q. Wang, R. H. Geng, and X. X. Zhang, “Analysis of chemical constituents in Artemisia argyi (Nanyang) by UPLC-Q-TOF-MS/MS,” Chinese Hospital Pharmacy Journal, vol. 38, no. 5, pp. 500–505, 2018. View at: Google Scholar
  13. D. D. Luo, H. S. Peng, Y. Zhang et al., “Comparison of chemical components between Artemisia stolonifera and Artemisia argyi using UPLC-Q-TOF-MS,” China Journal of Chinese Materia Medica, vol. 45, no. 17, pp. 4057–4064, 2020. View at: Google Scholar
  14. L. Li, L. Lv, X. Dong et al., “Rapid identification of chemical components in Artemisiae argyi folium by RRLC-TOFMS,” Journal of Pharmaceutical Practice, vol. 32, no. 6, pp. 448–452, 2014. View at: Google Scholar
  15. J.-X. Xia, B.-B. Zhao, J.-F. Zan, P. Wang, and L.-L. Chen, “Simultaneous determination of phenolic acids and flavonoids in Artemisiae Argyi Folium by HPLC-MS/MS and discovery of antioxidant ingredients based on relevance analysis,” Journal of Pharmaceutical and Biomedical Analysis, vol. 175, p. 112734, 2019. View at: Publisher Site | Google Scholar
  16. B. Zeng, H. M. Liu, X. M. Liu et al., “Application of UPLC-Q-exactive orbitrap MS technology in analysis of traditional Chinese medicine,” Journal of Chinese Medicinal Materials, vol. 43, no. 9, pp. 2313–2319, 2020. View at: Google Scholar
  17. H. J. Dong, X. H. Chen, and R. Zeng, “Rapid analysis on chemical constituents in roots of Rheum pumilum by UPLC coupled with hybrid quadrupole-orbit trap MS,” Chinese Traditional and Herbal Drugs, vol. 47, no. 14, pp. 2428–2435, 2016. View at: Google Scholar
  18. M. Gong, J. Q. Lu, and Y. S. Xiao, “Determination of total flavonoids and three main aglycones in folium Artemisiae argyi from different origins,” China Pharmacist, vol. 22, no. 5, pp. 966–968+975, 2019. View at: Google Scholar
  19. Q. Jiao, The Study of the Fingerprint and Multicomponent Content of Artemisia Argyi Folium and Artemisiae Lavandulaefoliae Folium, Hebei Medical University, Shijiazhuang, China, 2018.
  20. Q. Zhou, L. L. Sun, B. Jiang et al., “Simultaneous determination of eupatilin and jaceosidin in Artemisia argyi and its processed products by RP-HPLC,” China Pharmacy, vol. 24, no. 47, pp. 4464–4466, 2013. View at: Google Scholar
  21. L. Long, Y. J. Li, X. H. Cheng et al., “Determination of Eupatilin in Herb Artemisia,” Journal of Hubei University for Nationalities (Natural Science Edition), vol. 28, no. 4, pp. 395–396+404, 2010. View at: Google Scholar
  22. J. L. Wu, Y. L. Wang, W. Liu et al., “Simultaneous determination of 7 components in Artemisia argyi leaves by HPLC,” Chinese Traditional Patent Medicine, vol. 39, no. 9, pp. 1876–1879, 2017. View at: Google Scholar
  23. J. Wang, Z. Z. Han, L. W. Yang et al., “Comparison of UPLC and HPLC for the determination of 6,8,3',4'-tetramethoxy flavone in Murraya paniculate (L.) Jack,” Chinese Journal of Pharmaceutical Analysis, vol. 30, no. 8, pp. 1499–1501, 2010. View at: Google Scholar

Copyright © 2021 Lili Cui et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Related articles

No related content is available yet for this article.
 PDF Download Citation Citation
 Download other formatsMore
 Order printed copiesOrder

Related articles

No related content is available yet for this article.

Article of the Year Award: Outstanding research contributions of 2021, as selected by our Chief Editors. Read the winning articles.