ARTP Mutagenesis to Improve Mycelial Polysaccharide Production of Grifola frondosa Using a Mixture of Wheat Bran and Rice Bran as Substrate
Table 1
Uniform design of U11(113) parameters and results for GFA2 fermentation optimization. Each data point is the mean of three replicate samples.
Runs
Factors
Biomass Y1 (g/L)
Mycelial polysaccharides Y2 (g/L)
1x1
2x2
3x3
1
2.2727
2
2.25
21.61 ± 0.57
3.38 ± 0.17
2
9.909
0.25
2
18.33 ± 0.44
2.39 ± 0.13
3
1.1818
0.5
0.5
18.38 ± 1.16
2.42 ± 0.09
4
6.6363
1.25
2.5
20.76 ± 0.35
2.15 ± 0.12
5
5.5454
0
1
17.15 ± 1.38
1.72 ± 0.20
6
8.8181
1
0
19.40 ± 1.11
1.98 ± 0.06
7
0.0909
1.5
1.25
17.71 ± 2.12
2.44 ± 0.13
8
11
1.75
0.75
22.76 ± 1.18
3.15 ± 0.15
9
3.3636
0.75
1.75
21.10 ± 1.42
2.25 ± 0.22
10
7.7272
2.5
1.5
18.58 ± 0.74
1.80 ± 0.13
11
4.4545
2.25
0.25
19.27 ± 0.91
2.09 ± 0.06
1x1: the ratio of rice bran to wheat bran (total amount of rice and wheat brans was 120 g/L). 2x2: the concentration of KH2PO4. 3x3: the concentration of MgSO4·7H2O.
