Development of a New Nanocarrier for Dietary Garcinol: Characterization and In Vitro Efficacy Evaluation Using Breast Cancer Stem Cells Grown in Hypoxia

Table 2

Effects of variables (DMAB concentration, organic to aqueous phase volume ratio, and homogenization speed) on NP characteristics.

Parameters	Trial 1	Trial 2	Trial 3	Trial 4
DMAB concentration (mg/mL) (%)	1	1	0.5	1
Organic to aqueous phase volume ratio	1 : 2	1 : 4	1 : 4	1 : 4
Homogenization speed (rpm)	17,500	17,500	17,500	13,500
Particle size (nm)	127.6 ± 0.3	110.6 ± 1.2	151.9 ± 1.7	124.2 ± 0.7
PDI	0.072 ± 0.003	0.086 ± 0.01	0.047 ± 0.01	0.102 ± 0.003
Zeta potential (mV)	36.67 ± 0.9	39.63 ± 0.5	36.67 ± 0.9	38.67 ± 1.2