Isolation and Characterization of Gram-Negative Bacterial Species from Pasteurized Dairy Products: Potential Risk to Consumer Health

Figure 2

Multiplex PCR amplification results for detection of β-lactamase genes. Upper panel: lane 1: 1 Kb plus molecular weight marker; lane 2: positive control for SHV, CTX-M, and TEM with 739, 590, and 422 bp bands, respectively; lane 3: isolate 1 positive for both CTX-M and TEM with 590 and 422 bp bands; lane 4: isolate 3, positive for TEM (422 bp); lane 5: isolate 4, positive for both SHV and TEM with 739 and 422 bp; lanes 6–10: isolates 5, 6, 7, 9, and 12 all positive for TEM (422 bp). Lower panel: lanes 1 and 2: as in upper panel; lanes 3–5: isolates 15, 20, and 24 all positive for TEM (422 bp).