Comparison of Synergistic Effect of Nisin and Monolaurin on the Inactivation of Three Heat Resistant Spores Studied by Design of Experiments in Milk

Table 1

Experimental design for the central composite design.

Trial no.	Factors
	x1 (nisin (IU/ml))	x2 (monolaurin (µg/ml))	x3 (pH)
1	100	250	7
2	100	250	7
3	100	250	7
4	50	200	8
5	150	300	6
6	50	200	6
7	150	300	8
8	150	200	8
9	50	300	8
10	50	300	6
11	150	200	6
12	16	250	7
13	184	250	7
14	100	250	8.68
15	100	250	5.32
16	100	334	7
17	100	166	7