Optimising the (Microwave) Hydrothermal Pretreatment of Brewers Spent Grains for Bioethanol Production

Figure 2

The impact of pretreatment reaction temperature (110–200°C) and reaction time (1–10 min) on sugar yields following enzymatic saccharification of the BSG pretreatment solid residue. Treatment conditions varied according to D-optimal algorithm (see Table 2 for experimental design). Preliminary screening of autohydrolytical pretreatment of BSG at 2.5% (w/v) solids loading. 24 h enzyme incubation (50°C) using 200 mg residue to 40 mL of a 1 g/L Celluclast solution buffered to pH . Data are the mean ± SD of three replicate measurements.