Table of Contents
Journal of Histology
Volume 2013 (2013), Article ID 363857, 5 pages
http://dx.doi.org/10.1155/2013/363857
Research Article

Teratogenic Effects of Crude Ethanolic Root Bark and Leaf Extracts of Rauwolfia vomitoria (Apocynaceae) on the Femur of Albino Wistar Rat Fetuses

1Department of Anatomy, Faculty of Basic Medical Sciences, University of Calabar, PMB 1115 Calabar, Nigeria
2Department of Zoology and Environmental Biology, Faculty of Natural and Applied Sciences, University of Calabar, PMB 1115 Calabar, Nigeria
3Department of Anatomy, Faculty of Basic Medical Sciences, University of Uyo, PMB 1017 Uyo, Nigeria
4Department of Anatomy, Faculty of Basic Medical Sciences, Anambra State University, Uli, Nigeria

Received 4 April 2013; Revised 20 June 2013; Accepted 21 June 2013

Academic Editor: Francesco Cappello

Copyright © 2013 Mokutima A. Eluwa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Introduction. Rauwolfia vomitoria is a plant used as a sedative and in the treatment of psychotic tendency. This study was on the teratogenic effects of its root bark and leaf extracts on Wistar rat’s fetal femurs. Materials and Methods. Twenty-five female rats weighing between 180 and 200 g were divided into 5 groups, of 5 rats each. Group A was the control, while Groups B, C, D, and E were the experimental. The female rats were mated with mature male rats to allow for pregnancy. Groups B and C animals received orally 150 mg/kg each of the root bark and leaf extracts of Rauwolfia vomitoria, respectively, while Groups D and E animals received 250 mg/kg bodyweight each of the root bark and leaf extracts of Rauwolfia vomitoria, respectively, from day 7 to day 11 of gestation. On day 20 of gestation, the rats were sacrificed, the fetuses were examined, and their femurs were dissected out and preserved, decalcified, and routinely processed using the Haematoxylin and Eosin staining method. Results. Histological observations of the fetal femur bones showed numerous osteoblast and osteoclast, hypertrophy, and hyperplasia of bone cells compared with the control. Conclusion. Ethanolic root bark and leaf extracts of Rauwolfia vomitoria may lead to advanced skeletal development.