Table of Contents
Journal of Histology
Volume 2014, Article ID 130497, 6 pages
http://dx.doi.org/10.1155/2014/130497
Research Article

The Histochemical Characterization of the Glycoconjugates in the Epidermal Mucous Cells of the Red Californian Earthworm, Eisenia foetida

1Department of Biology, Faculty of Science and Art, Süleyman Demirel University, 32260 Isparta, Turkey
2Department of Biology, Faculty of Science and Art, Mehmet Akif Ersoy University, 15030 Burdur, Turkey

Received 9 June 2014; Revised 25 July 2014; Accepted 20 August 2014; Published 3 September 2014

Academic Editor: Salvatore Desantis

Copyright © 2014 Kenan Çinar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this study was to characterize the nature and regional distribution of the glycoconjugates secreted by epidermal mucous cells in Eisenia foetida (Annelida). Specimens were divided into six regions from anterior to posterior. The histochemistry was carried out by using standard histochemical methods. Histochemical staining properties of glycoconjugates in epidermal mucous cells were determined regionally. The epidermis of all regions contained strong to stronger PAS (+) cells in various degrees. The epidermis of the first, fourth, fifth, and sixth regions had strong to stronger AB pH 2.5 (+) cells. On the contrary, all regions contained weak to moderate AB pH 0.5 and AB pH 1.0 (+) cells. Most of mucous cells in epidermis of the first region contained both PAS (+) and AB (+) mucosubstances. All regions included weaker to weak AF (+) cells. All regions featured KOH/PAS (+) cells, with a slight reduction in reaction intensity in the epidermis of the last three regions. In this context, the different staining patterns observed in epidermal mucous cells hinted at their functional roles with respect to production of mucus with different physical properties. This study provided comprehensive information about the regional distribution patterns of the glycoconjugates and an opportunity to compare their distributional patterns in other annelids.