Table of Contents
Journal of Histology
Volume 2015 (2015), Article ID 718712, 10 pages
Research Article

Evaluation and Validation of a Semiautomatic Image Analysis Method: Quantification of Nares Cell Proliferation of Rhinella arenarum (Anura) Detected with the Avidin-Biotin Complex/Peroxidase, DAB

Microscopy Laboratory Applied to Molecular and Cellular Studies, School of Bioengineering, National University of Entre Ríos (UNER), Ruta 11 Km 10.5, Oro Verde, 3101 Entre Rios, Argentina

Received 8 September 2014; Accepted 9 February 2015

Academic Editor: Carlo Pellicciari

Copyright © 2015 Javier Eduardo Diaz Zamboni et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The current report presents the development and application of a novel methodological approach for computer-based methods of processing and analysis of proliferative tissues labeled by ABC-peroxidase method using 3, 3′-diaminobenzidine (DAB) as chromogen. This semiautomatic method is proposed to replace the classical manual approach, widely accepted as gold standard. Our method is based on a visual analysis of the microscopy image features from which a computational model is built to generate synthetic images which are used to evaluate and validate the methods of image processing and analysis. The evaluation allows knowing whether the computational methods applied are affected by the change of the image characteristics. Validation allows determining the method’s reliability and analyzing the concordance between the proposed method and a gold standard one. Additional strongness of this new approach is that it may be a framework adaptable to other studies made on any kind of microscopy.