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Journal of Healthcare Engineering
Volume 2017, Article ID 3050895, 6 pages
Research Article

Inhibitory Effect of Propolis on Platelet Aggregation In Vitro

1Department of Pathology, Weifang People’s Hospital, Weifang, Shandong Province 261041, China
2School of Pharmacy, Weifang Medical University, Weifang, Shandong Province 261053, China
3Department of Ophthalmology, Weifang Medical University, Weifang, Shandong Province 261053, China
4Department of Pathology and Pathophysiology, Taishan Medical University, Taian, Shandong Province 271016, China
5Departments of Haematology of University Medical Center, Utrecht University, 3508GA Utrecht, Netherlands

Correspondence should be addressed to Yun-Xiang Zhang; moc.361@295991gnibgnahz

Received 23 February 2017; Accepted 2 May 2017; Published 10 October 2017

Academic Editor: Wenxin Niu

Copyright © 2017 Yun-Xiang Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Platelet hyperactivity plays an important role in arterial thrombosis and atherosclerosis. The present study was aimed to investigate the effects of different extracts of propolis and components of flavonoids on platelet aggregation. Platelet-rich plasma was prepared and incubated in vitro with different concentrations of the tested extracts and components of flavonoids. Platelets aggregation was induced by different agonists including adenosine diphosphate (ADP, 10 μM), thrombin receptor activator peptide (TRAP, 50 μM), and collagen (5 μg/mL). At 25 mg/L to 300 mg/mL, the water extract propolis (WEP) inhibited three agonists-induced platelet aggregations in a dose-dependent manner. The flavonoids isolated from the propolis also showed markedly inhibited platelet aggregation induced by collagen, ADP, and TRAP, respectively. The components including caffeic acid phenethyl ester (CAPE), galangin, apigenin, quercetin, kaempferol, ferulic acid, rutin, chrysin, pinostrobin, and pinocembrin and their abilities of inhibiting platelet aggregation were studied. It was concluded that propolis had an antiplatelet action in which flavonoids were mainly implicated.