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Journal of Healthcare Engineering
Volume 2017, Article ID 4204085, 7 pages
Research Article

Model of Murine Ventricular Cardiac Tissue for In Vitro Kinematic-Dynamic Studies of Electromagnetic and β-Adrenergic Stimulation

1Department of Electrical, Computer and Biomedical Engineering, University of Pavia, Pavia, Italy
2Centre for Health Technologies (CHT), University of Pavia, Pavia, Italy
3IRCCS SDN, Naples, Italy
4Department of Experimental Medicine, Sapienza University of Rome, Rome, Italy
5Institute of Cell Biology and Neurobiology (IBCN), National Research Council (CNR), Rome, Italy
6Department of Anatomical, Histological, Forensic and Orthopaedic Sciences, Sapienza University of Rome, Rome, Italy

Correspondence should be addressed to Lorenzo Fassina; ti.vpinu@anissaf.oznerol

Received 13 February 2017; Accepted 17 July 2017; Published 8 August 2017

Academic Editor: Syoji Kobashi

Copyright © 2017 Lorenzo Fassina et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


In a model of murine ventricular cardiac tissue in vitro, we have studied the inotropic effects of electromagnetic stimulation (frequency, 75 Hz), isoproterenol administration (10 μM), and their combination. In particular, we have performed an image processing analysis to evaluate the kinematics and the dynamics of beating cardiac syncytia starting from the video registration of their contraction movement. We have found that the electromagnetic stimulation is able to counteract the β-adrenergic effect of isoproterenol and to elicit an antihypertrophic response.