miR-101-3p Contributes to α-Synuclein Aggregation in Neural Cells through the miR-101-3p/SKP1/PLK2 Pathway

Figure 3

SKP1 interact with PLK2 and promotes ubiquitination of PLK2. (a) SKP1 expression after SH-SY5Y was transfected by shRNA targeting SKP1. (b) Half-life of PLK2 evaluated after treated by cycloheximide. (c) Ubiquitination assay of PLK2 with or without SKP1 overexpression. (d) Western blot analysis of PLK2 after transfection of PLK2 overexpression plasmid. (e) Flow cytometry analysis of cell apoptosis.

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