[Retracted] COG133 Attenuates the Early Brain Injury Induced by Blood-Brain Barrier Disruption in Experimental Subarachnoid Hemorrhage

Figure 7

Pyroptosis of endothelial cells and astrocytes induced by NLRP3 inflammasome activation. (a–e) The expressions of NLRP3, pro-caspase-1, cleaved-caspase-1, ASC, and GSDMD were measured by western blotting assays. Results showed SAH induced the activation of the NLRP3 inflammasome, and COG133 inhibited NLRP3 inflammasome activation. (f, g) The levels of IL-1β and IL-18, which were related to the activation of the NLRP3 inflammasome, were detected by ELISA assays. Results showed COG133 suppressed the release of IL-1β and IL-18. (h, j) GSDMD/Lectin coimmunofluorescence staining showed that endothelial cells pyroptosis was induced by SAH and reduced by COG133. (i, k) GSDMD/GFAP coimmunofluorescence staining showed that astrocytes pyroptosis was promoted after SAH and COG133 treatment suppressed astrocytes pyroptosis.  < 0.05,  < 0.01,  < 0.001, and  < 0.0001 vs Sham group;  < 0.05,  < 0.01,  < 0.001, and  < 0.0001 vs SAH group.

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