MiR-98 Protects Nucleus Pulposus Cells against Apoptosis by Targeting TRAIL in Cervical Intervertebral Disc Degeneration

Figure 5

Overexpression of TRAIL reversed the inhibitory effects of miR-98 mimics on NP cell apoptosis stimulated by LPS. (a) Transfection efficiency of pcDNA-NC, pcDNA-TRAIL determined by qRT-PCR. (b) TRAIL protein levels determined by Western blot analysis in different groups (blank, LPS + pcDNA-NC, LPS + pcDNA-TRAIL, LPS + miR-98 mimics + pcDNA-NC, and LPS + miR-98 mimics + pcDNA-TRAIL). (c) The cell viability of NP cells performed by MTT assay in different groups (blank, LPS + pcDNA-NC, LPS + pcDNA-TRAIL, LPS + miR-98 mimics + pcDNA-NC, and LPS + miR-98 mimics + pcDNA-TRAIL). (d) NP cell apoptosis analyzed by flow cytometry in different groups (blank, LPS + pcDNA-NC, LPS + pcDNA-TRAIL, LPS + miR-98 mimics + pcDNA-NC, and LPS + miR-98 mimics + pcDNA-TRAIL). and ; and ; and .

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