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Developmental Immunology
Volume 2, Issue 2, Pages 85-94
http://dx.doi.org/10.1155/1992/45150

Analysis of Immature (CD4CD8) Thymic Subsets in T-Cell Receptor αß Transgenic Mice

1lnstitute of Pathology, University Hospital, Zurich 8091, Switzerland
2Ludwig Institute for Cancer Research, Lausanne Branch, Epalinges 1066, Switzerland

Copyright © 1992 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Introduction of a transgenic αß TCR (Vα2, Vß8.1) specific for lymphocytic choriomeningitis virus (LCMV), in the context of H-2Db into the genome of C57BL/6 mice, has many effects on the development and selection of T cells in both the thymus and the periphery. These mice produce increased numbers of CD48+ mature T cells, all of which express the transgenic TCR, and small numbers of CD48+cells using endogenous TCRs are also produced. This study follows the intrathymic development of T cells in these TCR αß transgenic mice, in particular the earliest CD48 stages. As expected, the transgenic TCR is expressed on the cell surface at an earlier developmental stage than endogenous TCRs in nontransgenic littermate controls. Of the three major subsets expressing the heat–stable antigen (HSA), only the most mature, the CD25CD44 expresses the transgenic TCR, and the earlier CD25CD44+ and CD25+CD44 do not. Furthermore, in contrast to other TCRαßtransgenic lines, TCR γδ lineage cells appear to develop normally.