About this Journal Submit a Manuscript Table of Contents
Clinical and Developmental Immunology
Volume 13 (2006), Issue 2-4, Pages 125-132
http://dx.doi.org/10.1080/17402520600877026

Towards a Vaccine Against Rheumatic Fever

1School of Medicine, Heart Institute (InCor), University of São Paulo, São Paulo, Brazil
2 Institute for Investigation in Immunology, Millennium Institute, São Paulo, Brazil
3Federal University of São Paulo (UNIFESP), São Paulo, Brazil
4Clinical Immunology and Allergy, Department of Clinical Medicine, School of Medicine, University of São Paulo, São Paulo, Brazil

Copyright © 2006 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Rheumatic fever (RF) is an autoimmune disease which affects more than 20 million children in developing countries. It is triggered by Streptococcus pyogenes throat infection in untreated susceptible individuals. Carditis, the most serious manifestation of the disease, leads to severe and permanent valvular lesions, causing chronic rheumatic heart disease (RHD). We have been studying the mechanisms leading to pathological autoimmunity in RF/RHD for the last 15 years. Our studies allowed us a better understanding of the cellular and molecular pathogenesis of RHD, paving the way for the development of a safe vaccine for a post-infection autoimmune disease. We have focused on the search for protective T and B cell epitopes by testing 620 human blood samples against overlapping peptides spanning 99 residues of the C-terminal portion of the M protein, differing by one amino acid residue. We identified T and B cell epitopes with 22 and 25 amino acid residues, respectively. Although these epitopes were from different regions of the C-terminal portion of the M protein, they showed an identical core of 16 amino acid residues. Antibodies against the B cell epitope inhibited bacterial invasion/adhesion in vitro. Our results strongly indicated that the selected T and B cell epitopes could potentially be protective against S. pyogenes.