Chronic Brucellosis Patients Retain Low Frequency of CD4+ T-Lymphocytes Expressing CD25 and CD28 after Escherichia coli LPS Stimulation of PHA-Cultured PBMCs

Table 3

Percentage of CD4+/CD25+, CD4+/CD28+ lymphocytes, and CD80+ cells of CD14+ population, in PHA-cultures with (a) E. coli LPS and (b) HKBA (results are presented as mean  ±  SD). NS: nonsignificant.

(a)	E. coli LPS cultures	n	%CD4+/CD25+	%CD4+/CD28+	%CD14+/CD80+
	AB	13	37.2±11.6	43.4±11.3	47.9±19.9
	CB	22	27.9±6.9	35.7±8.0	45.9±23.2
	Cured	11	30.8±12.8	39.8±17.2	21.8±14.4
	Controls	15	27.4±10.5	36.5±14.9	20.9±12.5
			P (AB versus cured)  =  NS	P (AB versus cured)  =  NS	P(AB versus cured)  =  .002
			P (AB versus controls)  =  .027	P (AB versus controls)  =  NS	P (AB versus controls)  <  .001
			P (CB versus cured)  =  NS	P (CB versus cured)  =  NS	P (CB versus cured)  =  .001
			P (CB versus controls)  =  NS	P (CB versus controls)  =  NS	P (CB versus controls)  <  .001
			P (AB versus CB)  =  .006	P (AB versus CB)  =  .024	P (AB versus CB)  =  NS
	CB1	12	26.3±5.9	34.5±8.7	49.9±26.5
	CB2	10	29.9±7.9	37.1±7.2	41.1±18.7
			P (CB1 versus AB)  =  .008	P (CB1 versus AB)  =  .039	P (CB1 versus CB2)  =  NS
					P (CB1 versus controls)  =  .003
					P (CB2 versus controls)  =  .004
(b)	HKBA cultures
	AB	13	22.4±11.5	35.9±14.5	30.0±20.8
	CB	22	24.8±6.5	38.8±11.2	36.5±25.5
	Cured	11	24.8±14.8	38.7±17.9	20.9±16.7
	Controls	15	20.6±10.3	35.6±16.9	16.3±13.6
			NS	NS	P (AB versus controls)  =  .047
					P (CB versus controls)  =  .004
	CB1	12	24.9±7.6	38.3±13.3	46.6±26.6
	CB2	10	24.7±5.1	39.3±8.7	24.4±18.7
			NS	NS	P (CB1 versus CB2)  =  .038
					P (CB1 versus cured)  =  .012
					P (CB1 versus controls)  =  .003