TY - JOUR A2 - Morimoto, Chikao AU - Olivo, R. A. AU - Nascimento, N. G. AU - Teixeira, C. F. P. AU - Silveira, P. F. PY - 2008 DA - 2008/02/25 TI - Methotrexate and Cyclosporine Treatments Modify the Activities of Dipeptidyl Peptidase IV and Prolyl Oligopeptidase in Murine Macrophages SP - 794050 VL - 2008 AB - Analysis of the effects of cyclosporine A (25–28 mgkg1) and/or methotrexate (0.1 mgkg1) treatments on dipeptidyl peptidase IV (DPPIV) and prolyl oligopeptidase (POP) activities and on algesic response in two distinct status of murine macrophages (Mφs) was undertaken. In resident Mφs, DPPIV and POP were affected by neither individual nor combined treatments. In thioglycolate-elicited Mφs, methotrexate increased DPPIV (99–110%) and POP (60%), while cyclosporine inhibited POP (21%). Combined treatment with both drugs promoted a rise (51–84%) of both enzyme activities. Only cyclosporine decreased (42%) the tolerance to algesic stimulus. Methotrexate was revealed to exert prevalent action over that of cyclosporine on proinflammatory Mφ POP. The opposite effects of methotrexate and cyclosporine on POP activity might influence the availability of the nociceptive mediators bradykinin and substance P in proinflammatory Mφs. The exacerbated response to thermally induced algesia observed in cyclosporine-treated animals could be related to upregulation of those mediators. SN - 2314-8861 UR - https://doi.org/10.1155/2008/794050 DO - 10.1155/2008/794050 JF - Clinical and Developmental Immunology PB - Hindawi Publishing Corporation KW - ER -