Journal of Immunology Research / 2009 / Article / Fig 5

Research Article

Immune Reactions Against Elongation Factor 2 Kinase: Specific Pathogenesis of Gastric Ulcer from Helicobacter pylori Infection

Figure 5

Flow cytometric analyses of target cells. (a) Typical analysis of EF-2K expression on HGC-27 cells is shown. The shadowed histogram indicates the EF-2K detected by GU sera in nontreated HGC-27 cells. And the bold histogram indicates the EF-2K detected by GU sera in heat-stressed HGC-27 cells. (b) Typical analysis of MHC class I molecule expression on HGC-27 cells is shown. The shadowed histogram indicates MHC class I molecules detected by PE-Cy5 conjugated mouse antihuman HLA-A, B, C antibody in nontreated HGC-27 cells and the bold histogram indicates MHC class I molecules detected by PE-Cy5 conjugated mouse antihuman HLA-A, B, C antibody in heat-stressed HGC-27 cells. The costimulatory molecules detected by FITC conjugated mouse antihuman CD80 antibody and PE conjugated mouse antihuman CD86 antibody on nontreated HGC-27 cells are shown in (c) and those of heat-stressed HGC-27 cells in (d). The heat stress enhanced the expression of EF-2K, MHC class I molecules, and costimulatory molecules on HGC-27 cells (target cells).
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(a)
850623.fig.005b
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(d)

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